Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01825 and PS417_01830 are separated by 87 nucleotidesPS417_01830 and PS417_01835 overlap by 4 nucleotidesPS417_01835 and PS417_01840 overlap by 4 nucleotidesPS417_01840 and PS417_01845 are separated by 10 nucleotides PS417_01825: PS417_01825 - chemotaxis protein, at 407,805 to 409,721 _01825 PS417_01830: PS417_01830 - 16S rRNA methyltransferase, at 409,809 to 410,516 _01830 PS417_01835: PS417_01835 - twin-arginine protein translocation system subunit TatC, at 410,513 to 411,301 _01835 PS417_01840: PS417_01840 - preprotein translocase, at 411,298 to 411,702 _01840 PS417_01845: PS417_01845 - preprotein translocase subunit SecA, at 411,713 to 411,991 _01845
Group Condition PS417_01825 PS417_01830 PS417_01835 PS417_01840 PS417_01845
rhizosphere rhizosphere sample 2; outgrowth in LB -1.3 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.3 N.D. N.D. N.D. N.D.
soil soil sample 5; outgrowth in LB -1.2 N.D. N.D. N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.2 N.D. N.D. N.D. N.D.
root root sample 6; outgrowth in LB -1.2 N.D. N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -1.1 N.D. N.D. N.D. N.D.
stress Tetracycline 0.001 mg/ml -1.1 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 N.D. N.D. N.D. N.D.
solid stress Fraxetin 2 mM; solid stress -1.1 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.0 N.D. N.D. N.D. N.D.
carbon source Glycerol (C) -1.0 N.D. N.D. N.D. N.D.
no phage control Time6-nophage -1.0 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.9 N.D. N.D. N.D. N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x -0.9 N.D. N.D. N.D. N.D.
carbon source RCH2_defined_glucose with MS_media 1x -0.9 N.D. N.D. N.D. N.D.
phage P. simiae OR Antrim MOI 10 -0.8 N.D. N.D. N.D. N.D.
motility_chemotaxis Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.8 N.D. N.D. N.D. N.D.
carbon source Inosine (C) +0.9 N.D. N.D. N.D. N.D.
carbon source L-Isoleucine (C) +0.9 N.D. N.D. N.D. N.D.
phage JP1 MOI 10 +1.0 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.0 N.D. N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.0 N.D. N.D. N.D. N.D.
phage JP1 MOI 1 +1.1 N.D. N.D. N.D. N.D.
phage Wcs_1 +1.1 N.D. N.D. N.D. N.D.
seeds Growth on radish seeds for 72 hours +1.2 N.D. N.D. N.D. N.D.
nophagecontrol Only library +1.2 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.3 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.3 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.4 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 N.D. N.D. N.D. N.D.
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