Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_18230 and PS417_18235 are separated by 73 nucleotidesPS417_18235 and PS417_18240 are separated by 41 nucleotidesPS417_18240 and PS417_18245 overlap by 4 nucleotidesPS417_18245 and PS417_18250 are separated by 18 nucleotides PS417_18230: PS417_18230 - amidase, at 3,972,172 to 3,973,686 _18230 PS417_18235: PS417_18235 - nitrile hydratase, at 3,973,760 to 3,974,353 _18235 PS417_18240: PS417_18240 - nitrile hydratase, at 3,974,395 to 3,975,057 _18240 PS417_18245: PS417_18245 - hypothetical protein, at 3,975,054 to 3,976,313 _18245 PS417_18250: PS417_18250 - chemotaxis protein, at 3,976,332 to 3,977,624 _18250
Group Condition PS417_18230 PS417_18235 PS417_18240 PS417_18245 PS417_18250
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 -0.9 -0.4 -0.1 -1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 -0.9 -0.2 -0.1 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.5 +0.2 -0.5 -1.6
seeds Growth on radish seeds for 24 hours -0.2 -0.9 -0.4 -0.1 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.5 +0.1 +0.2 -1.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.0 -1.4 -0.2 +0.2 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.6 -0.1 +0.1 -1.0
carbon source L-Leucine (C) -0.1 -1.1 -0.2 +0.2 -0.6
phage JP1 MOI 1 -0.4 +0.4 +0.0 -0.8 -1.0
seeds Growth on radish seeds for 72 hours +0.4 -1.2 +0.1 -0.2 -0.6
rhizosphere rhizosphere sample 2; outgrowth in LB -0.0 -1.5 +0.3 -0.0 -0.4
phage P. simiae ORA MOI 10 +0.3 -1.0 +0.2 -0.3 -0.7
soil soil sample 3; outgrowth in LB -0.1 -1.8 -0.3 +0.3 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.5 +0.4 +0.0 -0.1 -1.2
stress methylglyoxal 0.032 vol% +0.1 -0.7 +0.3 +0.1 -1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -1.0 +0.1 +0.2 -0.9
agar plate interaction control Taped volatile agar plate with no fungus -0.4 +0.5 -0.2 +0.3 -1.4
seeds Growth on radish seeds for 72 hours -0.4 -0.9 -0.4 +0.7 +0.2
motility inner cut, LB soft agar motility assay -0.3 +1.0 -0.3 -0.4 -0.6
phage P. simiae OR Antrim MOI 1 -0.2 +1.0 +0.1 -0.2 -1.0
phage JP1 MOI 10 -0.4 +0.8 -0.1 -0.6 +0.3
phage P. simiae Grant Run MOI 10 -0.4 +1.0 +0.1 -0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.9 -0.3 +0.2 +0.1 -0.7
phage P. simiae OR Antrim MOI 10 -0.5 +1.1 -0.2 -0.3 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.0 -0.6 +0.0 +0.1 +2.0
phage Wcs_0.1 -0.3 +1.1 -0.1 -0.1 +1.1
soil soil sample 5; outgrowth in LB +0.3 +0.7 -0.1 +0.4 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +2.1 +0.1 -0.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +1.3 +0.5 -0.1 +0.4
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 +1.7 -0.1 +0.2 +0.4
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