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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_18220 and PS417_18225 are separated by 83 nucleotides
PS417_18225 and PS417_18230 are separated by 189 nucleotides
PS417_18230 and PS417_18235 are separated by 73 nucleotides
PS417_18235 and PS417_18240 are separated by 41 nucleotides
PS417_18220: PS417_18220 - phenylacetaldoxime dehydratase, at 3,969,974 to 3,971,032
_18220
PS417_18225: PS417_18225 - phenol degradation protein meta, at 3,971,116 to 3,971,982
_18225
PS417_18230: PS417_18230 - amidase, at 3,972,172 to 3,973,686
_18230
PS417_18235: PS417_18235 - nitrile hydratase, at 3,973,760 to 3,974,353
_18235
PS417_18240: PS417_18240 - nitrile hydratase, at 3,974,395 to 3,975,057
_18240
Group
Condition
PS417
_18220
PS417
_18225
PS417
_18230
PS417
_18235
PS417
_18240
seeds
Growth on radish seeds for 72 hours
-0.0
-1.0
-0.4
-0.9
-0.4
soil
soil sample 3; outgrowth in LB
-0.5
-0.0
-0.1
-1.8
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
-0.2
-0.6
-0.9
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
+0.2
-0.6
-0.9
-0.2
seeds
Growth on radish seeds for 24 hours
+0.3
+0.1
-0.2
-0.9
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.4
-0.4
-0.9
-0.3
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.4
+0.6
-0.0
-1.5
+0.3
motility_chemotaxis
Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.4
+0.0
-0.4
-1.1
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
-0.0
+0.4
-1.0
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.4
-0.6
+0.2
-0.5
seeds
Growth on radish seeds for 72 hours
-0.2
+0.4
+0.4
-1.2
+0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.1
+0.3
-1.0
+0.5
-0.1
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.1
-0.5
-0.4
+0.8
-0.1
carbon source
2'-Deoxyinosine 5 mM (C)
-0.3
+0.6
+0.2
-0.8
+0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.5
-0.1
-0.3
+1.3
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.4
+0.3
-0.1
-0.8
+0.3
phage
P. simiae Grant Run MOI 10
+0.0
-0.4
-0.4
+1.0
+0.1
agar plate interaction control
Taped volatile agar plate with no fungus
+0.5
+0.3
-0.4
+0.5
-0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.4
-0.0
+0.9
-0.6
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.3
+1.0
-0.2
-0.4
-0.0
motility
inner cut, LB soft agar motility assay
-0.0
+0.3
-0.3
+1.0
-0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.3
-0.4
+0.9
-0.3
+0.2
soil
soil sample 4; outgrowth in LB
-0.2
+0.5
-0.4
+0.7
+0.3
phage
P. simiae OR Antrim MOI 10
+0.3
+0.6
-0.5
+1.1
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.3
-0.1
+1.4
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.3
+1.0
-0.2
+0.8
-0.1
phage
JP1 MOI 1
-0.1
+0.9
+0.1
+0.9
+0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.4
+0.1
-0.1
+2.1
+0.1
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.0
-0.1
+0.6
+1.7
-0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.0
+0.1
+0.2
+1.3
+0.5
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