Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_18220 and PS417_18225 are separated by 83 nucleotidesPS417_18225 and PS417_18230 are separated by 189 nucleotidesPS417_18230 and PS417_18235 are separated by 73 nucleotidesPS417_18235 and PS417_18240 are separated by 41 nucleotides PS417_18220: PS417_18220 - phenylacetaldoxime dehydratase, at 3,969,974 to 3,971,032 _18220 PS417_18225: PS417_18225 - phenol degradation protein meta, at 3,971,116 to 3,971,982 _18225 PS417_18230: PS417_18230 - amidase, at 3,972,172 to 3,973,686 _18230 PS417_18235: PS417_18235 - nitrile hydratase, at 3,973,760 to 3,974,353 _18235 PS417_18240: PS417_18240 - nitrile hydratase, at 3,974,395 to 3,975,057 _18240
Group Condition PS417_18220 PS417_18225 PS417_18230 PS417_18235 PS417_18240
seeds Growth on radish seeds for 72 hours -0.0 -1.0 -0.4 -0.9 -0.4
soil soil sample 3; outgrowth in LB -0.5 -0.0 -0.1 -1.8 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 -0.2 -0.6 -0.9 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.2 -0.6 -0.9 -0.2
seeds Growth on radish seeds for 24 hours +0.3 +0.1 -0.2 -0.9 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +0.4 -0.4 -0.9 -0.3
rhizosphere rhizosphere sample 2; outgrowth in LB -0.4 +0.6 -0.0 -1.5 +0.3
motility_chemotaxis Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.4 +0.0 -0.4 -1.1 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.0 +0.4 -1.0 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.4 -0.6 +0.2 -0.5
seeds Growth on radish seeds for 72 hours -0.2 +0.4 +0.4 -1.2 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 +0.3 -1.0 +0.5 -0.1
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.1 -0.5 -0.4 +0.8 -0.1
carbon source 2'-Deoxyinosine 5 mM (C) -0.3 +0.6 +0.2 -0.8 +0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 -0.1 -0.3 +1.3 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.3 -0.1 -0.8 +0.3
phage P. simiae Grant Run MOI 10 +0.0 -0.4 -0.4 +1.0 +0.1
agar plate interaction control Taped volatile agar plate with no fungus +0.5 +0.3 -0.4 +0.5 -0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -0.0 +0.9 -0.6 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 +1.0 -0.2 -0.4 -0.0
motility inner cut, LB soft agar motility assay -0.0 +0.3 -0.3 +1.0 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 -0.4 +0.9 -0.3 +0.2
soil soil sample 4; outgrowth in LB -0.2 +0.5 -0.4 +0.7 +0.3
phage P. simiae OR Antrim MOI 10 +0.3 +0.6 -0.5 +1.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.3 -0.1 +1.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 +1.0 -0.2 +0.8 -0.1
phage JP1 MOI 1 -0.1 +0.9 +0.1 +0.9 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +0.1 -0.1 +2.1 +0.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 -0.1 +0.6 +1.7 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +0.1 +0.2 +1.3 +0.5
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