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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_18175 and PS417_18180 are separated by 35 nucleotides
PS417_18180 and PS417_18185 are separated by 230 nucleotides
PS417_18185 and PS417_18190 are separated by 3 nucleotides
PS417_18190 and PS417_18195 are separated by 21 nucleotides
PS417_18175: PS417_18175 - MFS transporter, at 3,958,737 to 3,959,918
_18175
PS417_18180: PS417_18180 - dioxygenase, at 3,959,954 to 3,961,399
_18180
PS417_18185: PS417_18185 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 3,961,630 to 3,962,481
_18185
PS417_18190: PS417_18190 - IclR family transcriptional regulator, at 3,962,485 to 3,963,303
_18190
PS417_18195: PS417_18195 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 3,963,325 to 3,964,971
_18195
Group
Condition
PS417
_18175
PS417
_18180
PS417
_18185
PS417
_18190
PS417
_18195
motility
inner cut, LB soft agar motility assay
-0.5
-0.1
-0.3
-0.3
-0.8
motility
inner cut, LB soft agar motility assay
+0.0
-0.1
-0.7
-0.8
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.2
-0.4
-0.6
-0.7
phage
P. simiae OR1 MOI 0.1
-0.1
-0.1
-0.1
-0.9
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.2
-1.0
-0.2
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.2
-0.1
-1.0
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.1
-0.2
+0.1
-1.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.4
+0.2
-0.3
-0.8
-0.8
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.3
-0.3
+0.0
-0.5
-0.7
carbon source
L-Valine (C)
+0.1
+0.3
-0.3
-1.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
+0.2
-0.5
-0.9
+0.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.2
-0.0
+0.4
-0.2
-1.0
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
-0.6
-0.3
+0.3
+0.3
-0.2
carbon source
L-Valine (C)
+0.6
+0.1
-0.6
+0.4
-1.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.6
+0.3
-0.7
-0.3
-0.2
phage
JP1 MOI 10
+0.1
-0.2
+0.3
+0.5
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.2
-0.5
+0.7
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.4
-0.3
-0.4
+0.2
+1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.9
-0.2
-0.3
-0.4
+0.2
seeds
Growth on radish seeds for 72 hours
+0.7
-0.3
-0.2
+0.6
-0.5
soil
soil sample 5; outgrowth in LB
+0.0
-0.2
-0.8
+0.6
+0.8
phage
JP1 MOI 0.1
-0.1
-0.3
+0.7
+0.6
-0.2
phage
JP1 MOI 1
+0.2
-0.7
+0.2
-0.1
+1.0
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
-0.0
+1.0
-0.7
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.1
+0.3
-0.7
+1.1
+0.3
phage
P. simiae ORA MOI 0.1
-0.3
+0.1
+0.8
+0.3
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.2
-0.1
+0.5
+0.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.5
+0.6
-0.3
+0.0
+0.6
phage
JP1 MOI 10
+0.4
-0.5
+0.7
+0.5
+0.4
soil
soil sample 7; outgrowth in LB
+0.4
+0.3
+0.3
+0.6
-0.0
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