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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_18095 and PS417_18100 overlap by 4 nucleotides
PS417_18100 and PS417_18105 are separated by 2 nucleotides
PS417_18105 and PS417_18110 are separated by 224 nucleotides
PS417_18110 and PS417_18115 are separated by 84 nucleotides
PS417_18095: PS417_18095 - iron-dicitrate transporter subunit FecD, at 3,940,935 to 3,941,903
_18095
PS417_18100: PS417_18100 - iron ABC transporter, at 3,941,900 to 3,942,889
_18100
PS417_18105: PS417_18105 - iron-dicitrate transporter substrate-binding subunit, at 3,942,892 to 3,943,812
_18105
PS417_18110: PS417_18110 - TonB-dependent receptor, at 3,944,037 to 3,946,427
_18110
PS417_18115: PS417_18115 - gamma-glutamyltransferase, at 3,946,512 to 3,948,122
_18115
Group
Condition
PS417
_18095
PS417
_18100
PS417
_18105
PS417
_18110
PS417
_18115
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
+0.1
-0.1
-3.1
-0.3
-0.4
phage
Wcs_1
-0.0
-0.5
-1.5
-0.2
-0.9
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.0
-0.5
-2.2
-0.1
-0.1
soil
soil sample 4; outgrowth in LB
+0.3
-0.5
-2.7
+0.1
-0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.4
-0.1
-1.6
-0.1
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
+0.0
-1.2
-0.2
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
-0.2
-2.1
+0.2
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.1
-2.0
-0.3
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-1.2
+0.2
-1.0
-0.3
+0.0
supernatant control
Vogels_fungal_media 0.1X
-0.0
-0.1
-2.2
-0.0
+0.1
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
-0.3
-0.2
-1.3
+0.3
-0.6
carbon source
Carbon source D-Mannitol 10 mM
+0.0
+0.3
-2.3
+0.1
-0.1
seeds
Growth on radish seeds for 72 hours
+0.3
-1.2
-1.6
+0.2
+0.3
carbon source
Trisodium citrate (C)
-0.3
-0.4
-1.2
+0.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
-0.1
-0.6
-0.2
-1.0
solid stress
Fraxetin 3 mM; solid stress
+0.3
-0.0
-2.1
+0.3
+0.2
phage
JP1 MOI 1
+0.4
-0.5
-0.9
+0.2
-0.4
rhizosphere
rhizosphere sample 8; outgrowth in LB
-0.1
+0.3
-1.5
+0.5
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-0.4
+0.6
-0.3
-0.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.7
-0.4
+1.0
-0.2
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.4
+1.0
+0.2
-0.9
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.6
-0.6
-1.0
+0.7
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
+0.4
-0.9
+0.4
+0.2
agar plate interaction control
Taped volatile agar plate with no fungus
+0.9
-0.6
+0.2
-0.2
-0.5
phage
P. simiae OR Antrim MOI 0.1
-0.7
-0.6
+0.9
+0.3
+0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.6
+0.7
-0.7
+0.2
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
+0.3
+2.0
-0.2
-0.3
seeds
Growth on radish seeds for 72 hours
-0.8
+0.6
+1.0
+0.2
+0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.0
+0.4
+1.1
-0.1
+0.0
stress
Gentamicin 0.008 mg/ml
+0.4
+0.5
+1.2
+0.6
+0.0
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