Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_18080 and PS417_18085 overlap by 4 nucleotidesPS417_18085 and PS417_18090 are separated by 316 nucleotidesPS417_18090 and PS417_18095 are separated by 4 nucleotidesPS417_18095 and PS417_18100 overlap by 4 nucleotides PS417_18080: PS417_18080 - general secretion pathway protein GspF, at 3,936,938 to 3,938,134 _18080 PS417_18085: PS417_18085 - general secretion pathway protein GspE, at 3,938,131 to 3,939,822 _18085 PS417_18090: PS417_18090 - iron ABC transporter, at 3,940,139 to 3,940,930 _18090 PS417_18095: PS417_18095 - iron-dicitrate transporter subunit FecD, at 3,940,935 to 3,941,903 _18095 PS417_18100: PS417_18100 - iron ABC transporter, at 3,941,900 to 3,942,889 _18100
Group Condition PS417_18080 PS417_18085 PS417_18090 PS417_18095 PS417_18100
rhizosphere rhizosphere sample 2; outgrowth in LB -0.8 -0.1 -1.1 +0.2 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 -0.1 -1.2 -0.2 +0.3
seeds Growth on radish seeds for 72 hours +0.3 +0.3 -1.9 -0.2 +0.1
phage P. simiae OR Antrim MOI 0.1 +0.3 -0.1 -0.4 -0.8 -0.6
seeds Growth on radish seeds for 72 hours -0.2 -0.2 -0.6 -0.8 +0.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 +0.3 -0.4 +0.2 -1.0
phage Wcs_1 -0.2 +0.5 -0.7 -0.0 -0.5
seeds Growth on radish seeds for 72 hours +0.5 -0.3 -0.1 +0.3 -1.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 +0.5 -0.1 -0.7 -0.4
motility inner cut, LB soft agar motility assay +0.3 +0.2 -0.7 +0.3 -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 -0.1 -0.8 -0.5 +0.0
phage P. simiae OR Antrim MOI 1 +0.7 +0.1 -0.4 -0.8 -0.0
phage P. simiae ORA MOI 0.1 +0.5 -0.2 -0.8 +0.3 -0.3
phage JP1 MOI 1 -0.6 -0.3 +0.1 +0.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 +0.2 -0.1 +0.6 -0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 +0.3 +0.7 -0.6 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.9 -0.2 +0.6 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 +0.2 +0.6 +0.3 -0.4
agar plate interaction control Taped volatile agar plate with no fungus +0.1 -0.4 +0.2 +0.9 -0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.1 -0.7 +0.5 +0.7
carbon source L-Valine (C) +0.0 -0.3 -0.8 +0.9 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.8 +0.3 +0.9 +0.1 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.0 -0.3 +1.0 +0.4
carbon source 2'-Deoxyinosine 5 mM (C) +0.7 -0.1 -0.7 +0.5 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +0.0 +0.8 +0.5 +0.2
phage P. simiae OR Antrim MOI 10 +0.6 +0.3 +0.5 +0.2 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +1.0 +0.6 -0.5 +0.4
stress Gentamicin 0.008 mg/ml +0.4 -0.3 +0.6 +0.4 +0.5
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.7 +0.3 -0.1 +0.2 +0.6
phage JP1 MOI 0.1 +1.4 +0.2 +0.9 +0.6 -0.4
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