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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_18050 and PS417_18055 overlap by 4 nucleotides
PS417_18055 and PS417_18060 overlap by 4 nucleotides
PS417_18060 and PS417_18065 overlap by 4 nucleotides
PS417_18065 and PS417_18070 overlap by 4 nucleotides
PS417_18050: PS417_18050 - general secretion pathway protein GspL, at 3,933,520 to 3,934,512
_18050
PS417_18055: PS417_18055 - general secretion pathway protein GspK, at 3,934,509 to 3,935,210
_18055
PS417_18060: PS417_18060 - general secretion pathway protein GspJ, at 3,935,207 to 3,935,776
_18060
PS417_18065: PS417_18065 - general secretion pathway protein GspI, at 3,935,773 to 3,936,105
_18065
PS417_18070: PS417_18070 - general secretion pathway protein GspH, at 3,936,102 to 3,936,527
_18070
Group
Condition
PS417
_18050
PS417
_18055
PS417
_18060
PS417
_18065
PS417
_18070
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.8
+0.1
N.D.
-3.9
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-3.9
-0.6
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.1
-0.6
N.D.
-2.9
phage
P. simiae OR1 MOI 0.1
N.D.
-2.3
-0.7
N.D.
-1.1
phage
P. simiae ORA MOI 0.1
N.D.
-0.8
-0.8
N.D.
-1.6
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
N.D.
-1.6
-0.7
N.D.
-0.9
phage
P. simiae Grant Run MOI 10
N.D.
-2.5
+0.5
N.D.
-1.0
rhizosphere
rhizosphere sample 1; outgrowth in LB
N.D.
-0.7
-0.5
N.D.
-1.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.9
-0.6
N.D.
-1.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.3
-1.8
N.D.
-1.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-2.8
-0.5
N.D.
+0.8
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
N.D.
-1.6
+0.3
N.D.
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-2.2
-0.9
N.D.
+0.9
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-2.0
+0.3
N.D.
-0.5
soil
soil sample 4; outgrowth in LB
N.D.
-3.5
+0.6
N.D.
+0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.5
-1.0
N.D.
+1.1
phage
JP1 MOI 10
N.D.
-2.0
-0.4
N.D.
+1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.7
+0.2
N.D.
-2.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.9
-1.0
N.D.
+1.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-1.6
+0.3
N.D.
+1.2
carbon source
L-Valine (C)
N.D.
-1.4
+0.6
N.D.
+1.2
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
+2.2
+0.4
N.D.
-2.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.2
+0.8
N.D.
+0.9
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-1.2
+0.4
N.D.
+1.9
motility
inner cut, LB soft agar motility assay
N.D.
+1.4
-0.8
N.D.
+0.9
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
+1.1
+1.5
N.D.
-0.9
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.9
-0.0
N.D.
+2.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+1.1
+0.3
N.D.
+1.9
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+1.1
+0.5
N.D.
+1.8
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
+1.5
+1.0
N.D.
+1.3
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