Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_18050 and PS417_18055 overlap by 4 nucleotidesPS417_18055 and PS417_18060 overlap by 4 nucleotidesPS417_18060 and PS417_18065 overlap by 4 nucleotidesPS417_18065 and PS417_18070 overlap by 4 nucleotides PS417_18050: PS417_18050 - general secretion pathway protein GspL, at 3,933,520 to 3,934,512 _18050 PS417_18055: PS417_18055 - general secretion pathway protein GspK, at 3,934,509 to 3,935,210 _18055 PS417_18060: PS417_18060 - general secretion pathway protein GspJ, at 3,935,207 to 3,935,776 _18060 PS417_18065: PS417_18065 - general secretion pathway protein GspI, at 3,935,773 to 3,936,105 _18065 PS417_18070: PS417_18070 - general secretion pathway protein GspH, at 3,936,102 to 3,936,527 _18070
Group Condition PS417_18050 PS417_18055 PS417_18060 PS417_18065 PS417_18070
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.8 +0.1 N.D. -3.9
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -3.9 -0.6 N.D. -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.1 -0.6 N.D. -2.9
phage P. simiae OR1 MOI 0.1 N.D. -2.3 -0.7 N.D. -1.1
phage P. simiae ORA MOI 0.1 N.D. -0.8 -0.8 N.D. -1.6
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. -1.6 -0.7 N.D. -0.9
phage P. simiae Grant Run MOI 10 N.D. -2.5 +0.5 N.D. -1.0
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. -0.7 -0.5 N.D. -1.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.9 -0.6 N.D. -1.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.3 -1.8 N.D. -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.8 -0.5 N.D. +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -1.6 +0.3 N.D. -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -2.2 -0.9 N.D. +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -2.0 +0.3 N.D. -0.5
soil soil sample 4; outgrowth in LB N.D. -3.5 +0.6 N.D. +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.5 -1.0 N.D. +1.1
phage JP1 MOI 10 N.D. -2.0 -0.4 N.D. +1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.7 +0.2 N.D. -2.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.9 -1.0 N.D. +1.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.6 +0.3 N.D. +1.2
carbon source L-Valine (C) N.D. -1.4 +0.6 N.D. +1.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +2.2 +0.4 N.D. -2.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.2 +0.8 N.D. +0.9
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -1.2 +0.4 N.D. +1.9
motility inner cut, LB soft agar motility assay N.D. +1.4 -0.8 N.D. +0.9
carbon source 2'-Deoxyinosine 5 mM (C) N.D. +1.1 +1.5 N.D. -0.9
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.9 -0.0 N.D. +2.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.1 +0.3 N.D. +1.9
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.1 +0.5 N.D. +1.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +1.5 +1.0 N.D. +1.3
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