Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_18035 and PS417_18040 are separated by 5 nucleotidesPS417_18040 and PS417_18045 overlap by 4 nucleotidesPS417_18045 and PS417_18050 overlap by 26 nucleotidesPS417_18050 and PS417_18055 overlap by 4 nucleotides PS417_18035: PS417_18035 - general secretion pathway protein GspD, at 3,930,311 to 3,932,422 _18035 PS417_18040: PS417_18040 - general secretion pathway protein GspN, at 3,932,428 to 3,932,946 _18040 PS417_18045: PS417_18045 - general secretion pathway protein GspM, at 3,932,943 to 3,933,545 _18045 PS417_18050: PS417_18050 - general secretion pathway protein GspL, at 3,933,520 to 3,934,512 _18050 PS417_18055: PS417_18055 - general secretion pathway protein GspK, at 3,934,509 to 3,935,210 _18055
Group Condition PS417_18035 PS417_18040 PS417_18045 PS417_18050 PS417_18055
soil soil sample 4; outgrowth in LB +0.1 -0.5 N.D. N.D. -3.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.3 N.D. N.D. -3.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.7 N.D. N.D. -2.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.8 N.D. N.D. -2.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.2 -0.5 N.D. N.D. -2.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -1.0 N.D. N.D. -1.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 -0.4 N.D. N.D. -2.0
phage P. simiae Grant Run MOI 10 +0.1 -0.1 N.D. N.D. -2.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.2 N.D. N.D. -2.2
soil soil sample 5; outgrowth in LB -0.2 -1.2 N.D. N.D. -1.1
phage P. simiae Grant Run MOI 10 -0.2 -0.2 N.D. N.D. -2.1
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -0.1 -1.1 N.D. N.D. -1.2
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x +0.2 -1.3 N.D. N.D. -1.3
nophagecontrol Only library -0.1 -0.2 N.D. N.D. -2.0
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.2 -0.8 N.D. N.D. -1.6
carbon source Carbon source L-Citrulline 5 mM +0.2 -0.5 N.D. N.D. -1.8
phage JP1 MOI 10 +0.0 -0.2 N.D. N.D. -2.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x +0.3 -0.6 N.D. N.D. -1.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.3 N.D. N.D. -1.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 +0.3 N.D. N.D. -1.8
phage P. simiae OR1 MOI 0.1 -0.1 +0.8 N.D. N.D. -2.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.3 N.D. N.D. -2.0
solid stress Fraxetin 3 mM; solid stress -0.0 +0.8 N.D. N.D. -1.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.8 N.D. N.D. -1.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 +0.6 N.D. N.D. -1.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -1.7 N.D. N.D. +1.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 +2.4 N.D. N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.6 +0.9 N.D. N.D. +1.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 +0.9 N.D. N.D. +2.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 +5.8 N.D. N.D. -0.0
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