Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_17985 and PS417_17990 are separated by 163 nucleotidesPS417_17990 and PS417_17995 are separated by 180 nucleotidesPS417_17995 and PS417_18000 overlap by 22 nucleotidesPS417_18000 and PS417_18005 are separated by 97 nucleotides PS417_17985: PS417_17985 - ABC transporter ATP-binding protein, at 3,921,871 to 3,922,548 _17985 PS417_17990: PS417_17990 - hypothetical protein, at 3,922,712 to 3,923,425 _17990 PS417_17995: PS417_17995 - hypothetical protein, at 3,923,606 to 3,924,814 _17995 PS417_18000: PS417_18000 - hypothetical protein, at 3,924,793 to 3,925,365 _18000 PS417_18005: PS417_18005 - hypothetical protein, at 3,925,463 to 3,927,391 _18005
Group Condition PS417_17985 PS417_17990 PS417_17995 PS417_18000 PS417_18005
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 -0.8 -1.4 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.1 -0.9 -0.8 -0.1
soil soil sample 7; outgrowth in LB N.D. +0.0 -1.1 -0.7 -0.0
soil soil sample 5; outgrowth in LB N.D. -0.2 -0.6 -0.8 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.1 -1.2 -1.0 +0.6
stress Gentamicin 0.008 mg/ml N.D. +0.0 -1.1 -0.4 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.4 -1.1 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.3 -1.0 -0.2
supernatant control Vogels_fungal_media 0.1X N.D. -0.2 -1.1 +0.4 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.4 -0.7 +0.2
phage P. simiae OR1 MOI 0.1 N.D. -0.4 +0.6 -0.9 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 +0.8 -0.8 -0.0
soil soil sample 4; outgrowth in LB N.D. -0.2 +0.7 -0.6 -0.1
phage JP1 MOI 10 N.D. -0.0 -0.8 +0.8 -0.1
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.5 -0.4 +1.2 -0.2
carbon source 2-Deoxy-D-Ribose 10 mM (C) N.D. -0.1 -0.4 +1.0 -0.2
carbon source L-Valine (C) N.D. +0.5 +0.7 -0.8 +0.0
phage JP1 MOI 10 N.D. +0.0 -0.9 +1.1 +0.2
stress TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 N.D. -0.2 -0.4 +0.7 +0.2
seeds Growth on radish seeds for 72 hours N.D. -0.4 +0.6 -0.1 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.1 +0.8 -0.3 +0.3
seeds Growth on radish seeds for 72 hours N.D. -0.4 -0.0 +0.7 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.3 -0.3 +1.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.0 +0.4 +1.0 +0.0
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. +0.4 +0.5 +0.5 +0.1
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. +0.3 +0.4 +0.7 +0.0
stress Choline chloride 300 mM N.D. +0.4 -0.0 +1.0 +0.2
phage JP1 MOI 1 N.D. -0.0 +0.4 +1.1 +0.2
stress Nickel (II) chloride 1 mM N.D. +1.6 +0.4 -0.1 +0.0
stress Dimethyl Sulfoxide 5 vol% N.D. +0.3 +0.2 +1.6 +0.2
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