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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_17985 and PS417_17990 are separated by 163 nucleotides
PS417_17990 and PS417_17995 are separated by 180 nucleotides
PS417_17995 and PS417_18000 overlap by 22 nucleotides
PS417_18000 and PS417_18005 are separated by 97 nucleotides
PS417_17985: PS417_17985 - ABC transporter ATP-binding protein, at 3,921,871 to 3,922,548
_17985
PS417_17990: PS417_17990 - hypothetical protein, at 3,922,712 to 3,923,425
_17990
PS417_17995: PS417_17995 - hypothetical protein, at 3,923,606 to 3,924,814
_17995
PS417_18000: PS417_18000 - hypothetical protein, at 3,924,793 to 3,925,365
_18000
PS417_18005: PS417_18005 - hypothetical protein, at 3,925,463 to 3,927,391
_18005
Group
Condition
PS417
_17985
PS417
_17990
PS417
_17995
PS417
_18000
PS417
_18005
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.3
-0.8
-1.4
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.1
-0.9
-0.8
-0.1
soil
soil sample 7; outgrowth in LB
N.D.
+0.0
-1.1
-0.7
-0.0
soil
soil sample 5; outgrowth in LB
N.D.
-0.2
-0.6
-0.8
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.1
-1.2
-1.0
+0.6
stress
Gentamicin 0.008 mg/ml
N.D.
+0.0
-1.1
-0.4
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.2
-0.4
-1.1
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.2
-0.3
-1.0
-0.2
supernatant control
Vogels_fungal_media 0.1X
N.D.
-0.2
-1.1
+0.4
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.2
-0.4
-0.7
+0.2
phage
P. simiae OR1 MOI 0.1
N.D.
-0.4
+0.6
-0.9
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.2
+0.8
-0.8
-0.0
soil
soil sample 4; outgrowth in LB
N.D.
-0.2
+0.7
-0.6
-0.1
phage
JP1 MOI 10
N.D.
-0.0
-0.8
+0.8
-0.1
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
-0.5
-0.4
+1.2
-0.2
carbon source
2-Deoxy-D-Ribose 10 mM (C)
N.D.
-0.1
-0.4
+1.0
-0.2
carbon source
L-Valine (C)
N.D.
+0.5
+0.7
-0.8
+0.0
phage
JP1 MOI 10
N.D.
+0.0
-0.9
+1.1
+0.2
stress
TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
N.D.
-0.2
-0.4
+0.7
+0.2
seeds
Growth on radish seeds for 72 hours
N.D.
-0.4
+0.6
-0.1
+0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-0.1
+0.8
-0.3
+0.3
seeds
Growth on radish seeds for 72 hours
N.D.
-0.4
-0.0
+0.7
+0.4
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
N.D.
+0.3
-0.3
+1.0
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
N.D.
-0.0
+0.4
+1.0
+0.0
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
N.D.
+0.4
+0.5
+0.5
+0.1
stress
1-ethyl-3-methylimidazolium chloride 200 mM
N.D.
+0.3
+0.4
+0.7
+0.0
stress
Choline chloride 300 mM
N.D.
+0.4
-0.0
+1.0
+0.2
phage
JP1 MOI 1
N.D.
-0.0
+0.4
+1.1
+0.2
stress
Nickel (II) chloride 1 mM
N.D.
+1.6
+0.4
-0.1
+0.0
stress
Dimethyl Sulfoxide 5 vol%
N.D.
+0.3
+0.2
+1.6
+0.2
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