Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_17835 and PS417_17840 are separated by 5 nucleotidesPS417_17840 and PS417_17845 are separated by 49 nucleotidesPS417_17845 and PS417_17850 are separated by 15 nucleotidesPS417_17850 and PS417_17855 are separated by 63 nucleotides PS417_17835: PS417_17835 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 3,894,535 to 3,895,305 _17835 PS417_17840: PS417_17840 - betaine-aldehyde dehydrogenase, at 3,895,311 to 3,896,771 _17840 PS417_17845: PS417_17845 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 3,896,821 to 3,897,678 _17845 PS417_17850: PS417_17850 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 3,897,694 to 3,898,107 _17850 PS417_17855: PS417_17855 - major facilitator transporter, at 3,898,171 to 3,899,475 _17855
Group Condition PS417_17835 PS417_17840 PS417_17845 PS417_17850 PS417_17855
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 -1.0 -0.8 N.D. -0.6
soil soil sample 4; outgrowth in LB -0.9 -0.8 -0.6 N.D. -0.8
phage Wcs_1 -0.4 +0.0 -0.9 N.D. -1.4
soil soil sample 2; outgrowth in LB -1.1 -0.6 -0.4 N.D. -0.3
seeds Growth on radish seeds for 72 hours -0.3 -0.8 +0.2 N.D. -1.4
soil soil sample 6; outgrowth in LB +0.6 -1.9 +0.1 N.D. -1.1
rhizosphere rhizosphere sample 8; outgrowth in LB -0.1 -1.6 +0.2 N.D. -0.7
phage P. simiae OR Antrim MOI 0.1 -0.5 -0.9 +0.2 N.D. -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 -1.3 +0.5 N.D. -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.5 -1.1 +0.4 N.D. +0.2
stress 1-ethyl-3-methylimidazolium chloride 200 mM +0.3 -0.8 -0.4 N.D. -1.0
phage P. simiae Grant Run MOI 10 +0.4 -1.3 +0.2 N.D. -1.1
rhizosphere rhizosphere sample 2; outgrowth in LB -1.6 -0.2 +0.6 N.D. -0.2
soil soil sample 7; outgrowth in LB +0.8 -0.6 -0.3 N.D. -1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.7 +0.3 N.D. -1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -1.2 +0.6 N.D. -0.4
stress Nalidixic 0.015 mg/ml -1.4 +0.8 -0.1 N.D. +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.2 +1.1 +0.5 N.D. -0.7
phage moi 100 Time6-phageFRS -0.6 +0.6 -0.7 N.D. +0.7
phage JP1 MOI 0.1 +1.1 +0.5 -0.3 N.D. -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.3 -0.9 +0.2 N.D. +1.1
root root sample 6; outgrowth in LB +0.2 -0.5 -0.5 N.D. +1.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.7 +0.8 N.D. -1.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 +0.2 -0.5 N.D. +1.7
agar plate interaction control Taped volatile agar plate with no fungus +0.5 +1.4 +0.1 N.D. -0.8
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.8 -0.6 +0.3 N.D. +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 +1.8 +0.1 N.D. -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml +1.3 +0.4 +0.3 N.D. +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.7 +0.6 N.D. +0.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +1.0 -0.1 +0.1 N.D. +1.5
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