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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_17815 and PS417_17820 are separated by 149 nucleotides
PS417_17820 and PS417_17825 are separated by 206 nucleotides
PS417_17825 and PS417_17830 are separated by 128 nucleotides
PS417_17830 and PS417_17835 overlap by 4 nucleotides
PS417_17815: PS417_17815 - hypothetical protein, at 3,889,745 to 3,889,975
_17815
PS417_17820: PS417_17820 - hypothetical protein, at 3,890,125 to 3,892,677
_17820
PS417_17825: PS417_17825 - AraC family transcriptional regulator, at 3,892,884 to 3,893,744
_17825
PS417_17830: PS417_17830 - 4-hydroxyphenylacetate isomerase, at 3,893,873 to 3,894,538
_17830
PS417_17835: PS417_17835 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 3,894,535 to 3,895,305
_17835
Group
Condition
PS417
_17815
PS417
_17820
PS417
_17825
PS417
_17830
PS417
_17835
phage
P. simiae ORA MOI 0.1
N.D.
-0.0
-0.2
-1.3
-0.9
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
-0.3
-0.4
-1.2
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.2
-0.3
-2.0
-0.3
carbon source
Carbon source D-Mannitol 2.5 mM
N.D.
-0.1
-0.1
-1.0
-0.9
phage
P. simiae OR1 MOI 0.1
N.D.
+0.1
+0.1
-1.4
-0.7
no phage control
Time6-nophage
N.D.
+0.1
-0.3
-0.8
-0.9
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
+0.1
-0.0
-0.9
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.3
+0.1
-1.5
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.1
-0.5
-1.0
-0.1
rhizosphere
rhizosphere sample 2; outgrowth in LB
N.D.
+0.1
-0.1
-0.1
-1.6
phage
P. simiae OR Antrim MOI 10
N.D.
+0.1
-0.8
-0.4
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.0
+0.0
-0.2
-1.5
stress
Nalidixic 0.015 mg/ml
N.D.
-0.1
+0.1
-0.0
-1.4
supernatant control
Vogels_fungal_media; pH 5.5
N.D.
+0.1
+0.2
-0.6
-0.9
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
+0.5
-0.0
-1.9
+0.5
seeds
Growth on radish seeds for 72 hours
N.D.
+0.1
+0.6
-1.0
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
+0.3
+0.6
-0.4
-1.2
soil
soil sample 7; outgrowth in LB
N.D.
-0.0
-0.2
-1.2
+0.8
carbon source
D-Galacturonic Acid (C)
N.D.
+0.2
-0.2
-0.9
+0.3
soil
soil sample 2; outgrowth in LB
N.D.
-0.1
-0.2
+0.8
-1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
+0.0
+0.3
-1.1
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.4
+0.4
+0.5
-0.9
soil
soil sample 8; outgrowth in LB
N.D.
+0.2
+0.2
-1.0
+0.5
phage
JP1 MOI 1
N.D.
-0.0
+0.1
+1.0
-1.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
-0.2
-0.2
-0.5
+1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.2
-0.6
+0.3
+0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.2
-0.6
+0.4
+0.5
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
+0.3
-0.3
-0.3
+0.8
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.3
-0.3
+0.7
+0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-0.0
+0.6
-0.1
+1.3
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