Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_17815 and PS417_17820 are separated by 149 nucleotidesPS417_17820 and PS417_17825 are separated by 206 nucleotidesPS417_17825 and PS417_17830 are separated by 128 nucleotidesPS417_17830 and PS417_17835 overlap by 4 nucleotides PS417_17815: PS417_17815 - hypothetical protein, at 3,889,745 to 3,889,975 _17815 PS417_17820: PS417_17820 - hypothetical protein, at 3,890,125 to 3,892,677 _17820 PS417_17825: PS417_17825 - AraC family transcriptional regulator, at 3,892,884 to 3,893,744 _17825 PS417_17830: PS417_17830 - 4-hydroxyphenylacetate isomerase, at 3,893,873 to 3,894,538 _17830 PS417_17835: PS417_17835 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 3,894,535 to 3,895,305 _17835
Group Condition PS417_17815 PS417_17820 PS417_17825 PS417_17830 PS417_17835
phage P. simiae ORA MOI 0.1 N.D. -0.0 -0.2 -1.3 -0.9
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.3 -0.4 -1.2 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.3 -2.0 -0.3
carbon source Carbon source D-Mannitol 2.5 mM N.D. -0.1 -0.1 -1.0 -0.9
phage P. simiae OR1 MOI 0.1 N.D. +0.1 +0.1 -1.4 -0.7
no phage control Time6-nophage N.D. +0.1 -0.3 -0.8 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 -0.0 -0.9 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 +0.1 -1.5 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.1 -0.5 -1.0 -0.1
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. +0.1 -0.1 -0.1 -1.6
phage P. simiae OR Antrim MOI 10 N.D. +0.1 -0.8 -0.4 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.0 +0.0 -0.2 -1.5
stress Nalidixic 0.015 mg/ml N.D. -0.1 +0.1 -0.0 -1.4
supernatant control Vogels_fungal_media; pH 5.5 N.D. +0.1 +0.2 -0.6 -0.9
agar plate interaction control Taped volatile agar plate with no fungus N.D. +0.5 -0.0 -1.9 +0.5
seeds Growth on radish seeds for 72 hours N.D. +0.1 +0.6 -1.0 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.3 +0.6 -0.4 -1.2
soil soil sample 7; outgrowth in LB N.D. -0.0 -0.2 -1.2 +0.8
carbon source D-Galacturonic Acid (C) N.D. +0.2 -0.2 -0.9 +0.3
soil soil sample 2; outgrowth in LB N.D. -0.1 -0.2 +0.8 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.0 +0.3 -1.1 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 +0.4 +0.5 -0.9
soil soil sample 8; outgrowth in LB N.D. +0.2 +0.2 -1.0 +0.5
phage JP1 MOI 1 N.D. -0.0 +0.1 +1.0 -1.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -0.2 -0.2 -0.5 +1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 -0.6 +0.3 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.6 +0.4 +0.5
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. +0.3 -0.3 -0.3 +0.8
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.3 -0.3 +0.7 +0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.0 +0.6 -0.1 +1.3
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