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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_17650 and PS417_17655 are separated by 107 nucleotides
PS417_17655 and PS417_17660 are separated by 223 nucleotides
PS417_17660 and PS417_17665 are separated by 57 nucleotides
PS417_17665 and PS417_17670 are separated by 1 nucleotides
PS417_17650: PS417_17650 - proteinase, at 3,853,395 to 3,854,138
_17650
PS417_17655: PS417_17655 - GntR family transcriptional regulator, at 3,854,246 to 3,855,013
_17655
PS417_17660: PS417_17660 - branched-chain amino acid ABC transporter substrate-binding protein, at 3,855,237 to 3,856,439
_17660
PS417_17665: PS417_17665 - ABC transporter permease, at 3,856,497 to 3,857,357
_17665
PS417_17670: PS417_17670 - ABC transporter permease, at 3,857,359 to 3,858,369
_17670
Group
Condition
PS417
_17650
PS417
_17655
PS417
_17660
PS417
_17665
PS417
_17670
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
N.D.
-0.3
-0.5
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.0
N.D.
-0.1
+0.0
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.2
N.D.
+0.1
-0.0
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.7
N.D.
-0.4
+0.2
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.0
N.D.
-0.2
+0.3
-0.4
nitrogen source
Uridine (N)
+0.2
N.D.
-0.6
-0.3
-0.6
phage
P. simiae Grant Run MOI 0.1
-0.5
N.D.
+0.3
-0.5
-0.3
seeds
Growth on radish seeds for 72 hours
+0.4
N.D.
-0.3
-0.1
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.3
N.D.
+0.4
-0.2
+0.3
carbon source
L-Valine (C)
-1.4
N.D.
+0.3
-0.0
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
N.D.
+0.4
-0.6
-0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.7
N.D.
-0.6
-0.3
-0.6
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
-0.4
N.D.
-0.3
-0.5
+0.4
phage
P. simiae OR1 MOI 0.1
+0.5
N.D.
-0.1
-0.4
-0.8
motility
inner cut, LB soft agar motility assay
-0.4
N.D.
+0.5
-0.6
-0.2
soil
soil sample 5; outgrowth in LB
+0.7
N.D.
-0.5
+0.2
-1.0
stress
Gentamicin 0.008 mg/ml
-0.7
N.D.
+0.3
-0.4
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
N.D.
+0.3
+0.5
-0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.3
N.D.
+0.4
+0.3
-0.9
soil
soil sample 3; outgrowth in LB
+0.5
N.D.
+0.4
-0.1
-0.6
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
-0.7
N.D.
+0.2
+0.4
+0.5
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
-0.3
N.D.
+0.2
-0.2
+0.9
rhizosphere
rhizosphere sample 1; outgrowth in LB
+0.6
N.D.
-0.1
-0.4
+0.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.0
N.D.
+0.3
+0.3
-0.5
carbon source
L-Valine (C)
-0.1
N.D.
+0.4
-0.1
+1.0
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.3
N.D.
+1.0
+0.6
+0.3
agar plate interaction control
Taped volatile agar plate with no fungus
+0.4
N.D.
+0.7
-0.2
+0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.5
N.D.
-0.1
+0.2
+1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
N.D.
+0.5
+0.5
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+3.4
N.D.
-0.2
+0.4
-0.1
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