Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_17650 and PS417_17655 are separated by 107 nucleotidesPS417_17655 and PS417_17660 are separated by 223 nucleotidesPS417_17660 and PS417_17665 are separated by 57 nucleotidesPS417_17665 and PS417_17670 are separated by 1 nucleotides PS417_17650: PS417_17650 - proteinase, at 3,853,395 to 3,854,138 _17650 PS417_17655: PS417_17655 - GntR family transcriptional regulator, at 3,854,246 to 3,855,013 _17655 PS417_17660: PS417_17660 - branched-chain amino acid ABC transporter substrate-binding protein, at 3,855,237 to 3,856,439 _17660 PS417_17665: PS417_17665 - ABC transporter permease, at 3,856,497 to 3,857,357 _17665 PS417_17670: PS417_17670 - ABC transporter permease, at 3,857,359 to 3,858,369 _17670
Group Condition PS417_17650 PS417_17655 PS417_17660 PS417_17665 PS417_17670
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 N.D. -0.3 -0.5 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.0 N.D. -0.1 +0.0 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.2 N.D. +0.1 -0.0 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.7 N.D. -0.4 +0.2 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 N.D. -0.2 +0.3 -0.4
nitrogen source Uridine (N) +0.2 N.D. -0.6 -0.3 -0.6
phage P. simiae Grant Run MOI 0.1 -0.5 N.D. +0.3 -0.5 -0.3
seeds Growth on radish seeds for 72 hours +0.4 N.D. -0.3 -0.1 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.3 N.D. +0.4 -0.2 +0.3
carbon source L-Valine (C) -1.4 N.D. +0.3 -0.0 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 N.D. +0.4 -0.6 -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.7 N.D. -0.6 -0.3 -0.6
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 N.D. -0.3 -0.5 +0.4
phage P. simiae OR1 MOI 0.1 +0.5 N.D. -0.1 -0.4 -0.8
motility inner cut, LB soft agar motility assay -0.4 N.D. +0.5 -0.6 -0.2
soil soil sample 5; outgrowth in LB +0.7 N.D. -0.5 +0.2 -1.0
stress Gentamicin 0.008 mg/ml -0.7 N.D. +0.3 -0.4 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 N.D. +0.3 +0.5 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. +0.4 +0.3 -0.9
soil soil sample 3; outgrowth in LB +0.5 N.D. +0.4 -0.1 -0.6
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) -0.7 N.D. +0.2 +0.4 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.3 N.D. +0.2 -0.2 +0.9
rhizosphere rhizosphere sample 1; outgrowth in LB +0.6 N.D. -0.1 -0.4 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 N.D. +0.3 +0.3 -0.5
carbon source L-Valine (C) -0.1 N.D. +0.4 -0.1 +1.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.3 N.D. +1.0 +0.6 +0.3
agar plate interaction control Taped volatile agar plate with no fungus +0.4 N.D. +0.7 -0.2 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.5 N.D. -0.1 +0.2 +1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 N.D. +0.5 +0.5 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +3.4 N.D. -0.2 +0.4 -0.1
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