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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_17615 and PS417_17620 overlap by 4 nucleotides
PS417_17620 and PS417_17625 are separated by 77 nucleotides
PS417_17625 and PS417_17630 are separated by 119 nucleotides
PS417_17630 and PS417_17635 are separated by 365 nucleotides
PS417_17615: PS417_17615 - leucyl-tRNA synthetase, at 3,844,323 to 3,845,153
_17615
PS417_17620: PS417_17620 - biotin synthase, at 3,845,150 to 3,846,370
_17620
PS417_17625: PS417_17625 - ornithine monooxygenase, at 3,846,448 to 3,847,785
_17625
PS417_17630: PS417_17630 - RNA polymerase sigma factor, at 3,847,905 to 3,848,387
_17630
PS417_17635: PS417_17635 - hemolysin secretion protein D, at 3,848,753 to 3,849,922
_17635
Group
Condition
PS417
_17615
PS417
_17620
PS417
_17625
PS417
_17630
PS417
_17635
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.8
-0.0
+1.4
-0.7
-3.0
stress
Cisplatin 0.01 mg/ml
-1.0
-1.0
-0.2
-0.3
+0.1
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.9
-0.2
+0.2
-1.2
-0.2
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
-0.3
-0.0
+1.9
-1.1
-1.8
soil
soil sample 2; outgrowth in LB
+0.2
+0.2
+0.1
-1.1
-0.3
phage
JP1 MOI 10
+0.3
-0.1
-0.1
-1.6
+0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.5
+0.1
-0.3
-0.7
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
+0.3
+0.5
-1.7
-0.2
phage
FRSphage
+0.4
+0.0
-0.3
-1.3
+0.7
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.3
-0.0
+0.1
-1.1
+0.3
solid stress
Fraxetin 3 mM; solid stress
-0.5
+0.1
-0.2
-0.4
+0.7
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.5
+0.1
-0.5
-0.6
motility
inner cut, LB soft agar motility assay
+0.6
-0.0
+0.4
-0.9
-0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.7
-0.2
+0.6
-1.2
+0.0
phage moi 100
Time6-phageFRS
+0.4
+0.2
-0.1
-1.0
+0.4
rhizosphere
rhizosphere sample 1; outgrowth in LB
+0.4
+0.3
+0.3
-0.7
-0.2
stress
methylglyoxal 0.032 vol%
-0.9
+0.4
-0.2
+0.0
+1.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.3
+0.3
+0.3
-0.4
+0.5
seeds
Growth on radish seeds for 72 hours
+0.1
+0.5
+0.1
-0.7
+0.5
seeds
Growth on radish seeds for 72 hours
+0.8
+0.0
+0.2
-0.6
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
-0.1
-0.0
-0.5
+1.3
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
+0.1
+0.3
-0.3
+1.0
-0.2
phage
P. simiae ORA MOI 0.1
+1.0
-0.3
-0.2
+0.6
-0.3
soil
soil sample 7; outgrowth in LB
+0.3
-0.4
+0.3
+0.2
+0.5
phage
P. simiae Grant Run MOI 10
+0.0
+0.2
+0.0
+1.2
-0.4
soil
soil sample 5; outgrowth in LB
+0.9
-0.2
-0.1
+0.6
+0.1
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
-0.2
+0.3
+0.3
+1.1
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.3
+0.2
+0.2
+1.1
+0.1
carbon source
2'-Deoxyinosine 5 mM (C)
+0.4
-0.1
+0.1
+0.6
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
+0.0
+0.2
+1.3
+0.4
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