Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_17390 and PS417_17395 overlap by 4 nucleotidesPS417_17395 and PS417_17400 are separated by 116 nucleotidesPS417_17400 and PS417_17405 are separated by 177 nucleotidesPS417_17405 and PS417_17410 are separated by 184 nucleotides PS417_17390: PS417_17390 - DSBA oxidoreductase, at 3,798,149 to 3,799,294 _17390 PS417_17395: PS417_17395 - MFS transporter, at 3,799,291 to 3,800,934 _17395 PS417_17400: PS417_17400 - TetR family transcriptional regulator, at 3,801,051 to 3,801,638 _17400 PS417_17405: PS417_17405 - AraC family transcriptional regulator, at 3,801,816 to 3,802,604 _17405 PS417_17410: PS417_17410 - membrane protein, at 3,802,789 to 3,804,267 _17410
Group Condition PS417_17390 PS417_17395 PS417_17400 PS417_17405 PS417_17410
carbon source octanoate (C) -3.6 -3.2 +0.5 +0.2 +0.3
root root sample 6; outgrowth in LB +0.2 +0.2 -0.3 -4.5 -0.3
phage Wcs_1 -0.7 +0.2 -1.3 -2.6 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.4 -1.3 -1.7 -0.8
carbon source octanoate (C) -2.5 -2.7 +1.0 +0.0 +0.5
carbon source 2'-Deoxyinosine 5 mM (C) -0.3 -0.2 -0.0 -2.0 -0.5
seeds Growth on radish seeds for 72 hours -0.0 -0.1 -0.1 -2.0 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +0.1 -0.2 -1.3 -0.7
carbon source L-Valine (C) -0.8 -0.6 +0.4 -1.1 -0.0
stress Gentamicin 0.008 mg/ml -1.1 -0.2 -0.4 +0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.4 +0.5 -1.7 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 -0.1 +0.2 -0.2 -0.9
soil soil sample 7; outgrowth in LB +0.3 -0.1 +0.1 -1.1 -0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -0.1 +0.2 -1.4 -0.3
phage JP1 MOI 1 -0.8 +0.4 -0.4 -0.7 -0.0
seeds Growth on radish seeds for 72 hours +0.4 +0.3 +0.3 -2.1 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.2 -0.9 +0.7 +0.2
rhizosphere rhizosphere sample 2; outgrowth in LB +0.2 -0.5 -0.0 +0.9 -0.7
agar plate interaction control Taped volatile agar plate with no fungus -0.9 -0.2 +0.2 +0.7 +0.1
phage P. simiae OR Antrim MOI 10 +0.1 +0.2 +0.4 -1.0 +0.5
stress 1-ethyl-3-methylimidazolium chloride 200 mM -0.4 -0.1 +0.7 +0.7 -0.3
motility outer cut, LB soft agar motility assay -0.1 +0.2 +0.2 +0.9 -0.7
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.4 +0.0 -0.0 +1.6 -0.5
phage P. simiae OR Antrim MOI 0.1 -0.1 -0.3 -0.3 +1.7 -0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -0.0 +0.4 +0.8 -0.4
phage P. simiae ORA MOI 10 +0.1 +0.1 -0.4 +0.9 +0.7
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.6 +1.1 -1.2 +0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 +0.4 +0.4 +1.0 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 +0.8 -0.3 +0.8 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.8 +0.1 +1.0 +1.4 +0.0
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