Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_17235 and PS417_17240 are separated by 91 nucleotidesPS417_17240 and PS417_17245 are separated by 401 nucleotidesPS417_17245 and PS417_17250 are separated by 77 nucleotidesPS417_17250 and PS417_17255 are separated by 56 nucleotides PS417_17235: PS417_17235 - Clp protease ClpP, at 3,766,717 to 3,767,352 _17235 PS417_17240: PS417_17240 - trigger factor, at 3,767,444 to 3,768,754 _17240 PS417_17245: PS417_17245 - tRNA-His, at 3,769,156 to 3,769,231 _17245 PS417_17250: PS417_17250 - tRNA-Leu, at 3,769,309 to 3,769,393 _17250 PS417_17255: PS417_17255 - tRNA-His, at 3,769,450 to 3,769,525 _17255
Group Condition PS417_17235 PS417_17240 PS417_17245 PS417_17250 PS417_17255
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. -4.4 N.D. N.D. N.D.
phage P. simiae OR Antrim MOI 10 N.D. -3.2 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -3.1 N.D. N.D. N.D.
soil soil sample 6; outgrowth in LB N.D. -3.0 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -2.6 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -2.5 N.D. N.D. N.D.
agar plate interaction control Taped volatile agar plate with no fungus N.D. -2.5 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -2.5 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -2.4 N.D. N.D. N.D.
phage P. simiae OR Antrim MOI 1 N.D. -2.4 N.D. N.D. N.D.
phage P. simiae OR Antrim MOI 0.1 N.D. -2.4 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. -2.3 N.D. N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 N.D. -2.3 N.D. N.D. N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. -2.2 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. -2.1 N.D. N.D. N.D.
phage P. simiae OR Antrim MOI 1 N.D. -2.1 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay N.D. -2.0 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB N.D. -2.0 N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay N.D. -1.9 N.D. N.D. N.D.
phage P. simiae ORA MOI 1 N.D. -1.9 N.D. N.D. N.D.
soil soil sample 7; outgrowth in LB N.D. -1.9 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -1.8 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.8 N.D. N.D. N.D.
ecofab LB 0.5x in EcoFAB_3.5mL N.D. -1.8 N.D. N.D. N.D.
stress Choline chloride 300 mM N.D. -1.8 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -1.8 N.D. N.D. N.D.
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) N.D. -1.8 N.D. N.D. N.D.
soil soil sample 2; outgrowth in LB N.D. -1.7 N.D. N.D. N.D.
phage JP1 MOI 10 N.D. -1.7 N.D. N.D. N.D.
stress methylglyoxal 0.032 vol% N.D. +1.8 N.D. N.D. N.D.
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