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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_17235 and PS417_17240 are separated by 91 nucleotides
PS417_17240 and PS417_17245 are separated by 401 nucleotides
PS417_17245 and PS417_17250 are separated by 77 nucleotides
PS417_17250 and PS417_17255 are separated by 56 nucleotides
PS417_17235: PS417_17235 - Clp protease ClpP, at 3,766,717 to 3,767,352
_17235
PS417_17240: PS417_17240 - trigger factor, at 3,767,444 to 3,768,754
_17240
PS417_17245: PS417_17245 - tRNA-His, at 3,769,156 to 3,769,231
_17245
PS417_17250: PS417_17250 - tRNA-Leu, at 3,769,309 to 3,769,393
_17250
PS417_17255: PS417_17255 - tRNA-His, at 3,769,450 to 3,769,525
_17255
Group
Condition
PS417
_17235
PS417
_17240
PS417
_17245
PS417
_17250
PS417
_17255
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
-4.4
N.D.
N.D.
N.D.
phage
P. simiae OR Antrim MOI 10
N.D.
-3.2
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-3.1
N.D.
N.D.
N.D.
soil
soil sample 6; outgrowth in LB
N.D.
-3.0
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-2.6
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-2.5
N.D.
N.D.
N.D.
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
-2.5
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-2.5
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-2.4
N.D.
N.D.
N.D.
phage
P. simiae OR Antrim MOI 1
N.D.
-2.4
N.D.
N.D.
N.D.
phage
P. simiae OR Antrim MOI 0.1
N.D.
-2.4
N.D.
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
N.D.
-2.3
N.D.
N.D.
N.D.
supernatant control
Vogels_fungal_media; pH 5.5
N.D.
-2.3
N.D.
N.D.
N.D.
rhizosphere
rhizosphere sample 1; outgrowth in LB
N.D.
-2.2
N.D.
N.D.
N.D.
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
N.D.
-2.1
N.D.
N.D.
N.D.
phage
P. simiae OR Antrim MOI 1
N.D.
-2.1
N.D.
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
N.D.
-2.0
N.D.
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB
N.D.
-2.0
N.D.
N.D.
N.D.
motility
inner cut, LB soft agar motility assay
N.D.
-1.9
N.D.
N.D.
N.D.
phage
P. simiae ORA MOI 1
N.D.
-1.9
N.D.
N.D.
N.D.
soil
soil sample 7; outgrowth in LB
N.D.
-1.9
N.D.
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB
N.D.
-1.8
N.D.
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-1.8
N.D.
N.D.
N.D.
ecofab
LB 0.5x in EcoFAB_3.5mL
N.D.
-1.8
N.D.
N.D.
N.D.
stress
Choline chloride 300 mM
N.D.
-1.8
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
-1.8
N.D.
N.D.
N.D.
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
N.D.
-1.8
N.D.
N.D.
N.D.
soil
soil sample 2; outgrowth in LB
N.D.
-1.7
N.D.
N.D.
N.D.
phage
JP1 MOI 10
N.D.
-1.7
N.D.
N.D.
N.D.
stress
methylglyoxal 0.032 vol%
N.D.
+1.8
N.D.
N.D.
N.D.
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