Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_17080 and PS417_17085 overlap by 10 nucleotidesPS417_17085 and PS417_17090 are separated by 73 nucleotidesPS417_17090 and PS417_17095 are separated by 25 nucleotidesPS417_17095 and PS417_17100 are separated by 2 nucleotides PS417_17080: PS417_17080 - lipoprotein, at 3,730,617 to 3,731,597 _17080 PS417_17085: PS417_17085 - fimbrial assembly protein, at 3,731,588 to 3,733,948 _17085 PS417_17090: PS417_17090 - molecular chaperone, at 3,734,022 to 3,734,810 _17090 PS417_17095: PS417_17095 - spore coat protein, at 3,734,836 to 3,735,324 _17095 PS417_17100: PS417_17100 - spore coat protein, at 3,735,327 to 3,735,869 _17100
Group Condition PS417_17080 PS417_17085 PS417_17090 PS417_17095 PS417_17100
soil soil sample 5; outgrowth in LB -0.6 -0.3 N.D. -2.4 -0.3
phage P. simiae ORA MOI 1 +0.1 +0.2 N.D. -0.7 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.2 -0.2 N.D. -0.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.1 +0.2 N.D. +0.1 -0.9
solid stress Fraxetin 3 mM; solid stress +0.3 +0.1 N.D. -0.6 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.0 N.D. -0.4 -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.5 -0.1 N.D. +0.5 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.1 N.D. -0.7 +0.2
phage P. simiae OR Antrim MOI 10 +0.3 +0.1 N.D. -0.9 +0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.0 +0.2 N.D. +0.5 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.2 +0.3 N.D. +0.2 -0.7
carbon source 2'-Deoxyinosine 5 mM (C) -0.2 -0.2 N.D. +0.5 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.2 +0.4 N.D. -0.4 +0.9
phage P. simiae Grant Run MOI 10 -0.0 +0.1 N.D. +1.0 -0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 -0.1 N.D. +1.2 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.5 +0.1 N.D. -0.3 +0.5
phage P. simiae Grant Run MOI 0.1 -0.2 -0.1 N.D. +0.9 +0.3
stress Gentamicin 0.008 mg/ml +0.1 +0.3 N.D. +0.8 -0.1
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 +0.7 N.D. -0.3 +0.3
phage P. simiae OR1 MOI 0.1 -0.3 +0.1 N.D. +1.3 +0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.0 +0.1 N.D. +0.4 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.0 N.D. +0.8 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +0.4 N.D. +0.3 +0.7
phage P. simiae OR Antrim MOI 1 +0.2 +0.2 N.D. +0.6 +0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.2 +0.1 N.D. +0.9 +0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.4 N.D. +0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 +0.2 N.D. +0.1 +0.9
stress R2A with Fusaric 120 ug/mL +0.2 +0.2 N.D. +1.0 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.1 N.D. +0.1 +1.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.5 N.D. +0.3 +1.0
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