Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_17000 and PS417_17005 are separated by 16 nucleotidesPS417_17005 and PS417_17010 are separated by 681 nucleotidesPS417_17010 and PS417_17015 are separated by 151 nucleotidesPS417_17015 and PS417_17020 are separated by 11 nucleotides PS417_17000: PS417_17000 - MerR family transcriptional regulator, at 3,713,796 to 3,714,188 _17000 PS417_17005: PS417_17005 - hydroxymethylglutaryl-CoA lyase, at 3,714,205 to 3,715,104 _17005 PS417_17010: PS417_17010 - AMP-binding protein, at 3,715,786 to 3,717,420 _17010 PS417_17015: PS417_17015 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (from data), at 3,717,572 to 3,718,735 _17015 PS417_17020: PS417_17020 - methylcrotonoyl-CoA carboxylase, at 3,718,747 to 3,720,354 _17020
Group Condition PS417_17000 PS417_17005 PS417_17010 PS417_17015 PS417_17020
phage JP1 MOI 1 -3.6 N.D. -0.9 -0.2 N.D.
phage JP1 MOI 10 -4.7 N.D. -0.5 +0.5 N.D.
carbon source L-Valine (C) -3.2 N.D. -0.5 +0.4 N.D.
phage P. simiae OR Antrim MOI 10 -2.2 N.D. -0.6 -0.3 N.D.
carbon source L-Leucine (C) +0.5 N.D. +0.3 -3.9 N.D.
phage JP1 MOI 1 -2.7 N.D. -0.5 +0.1 N.D.
phage JP1 MOI 10 -2.5 N.D. -0.8 +0.2 N.D.
carbon source L-Leucine (C) +0.0 N.D. +0.1 -3.1 N.D.
phage P. simiae OR Antrim MOI 1 -1.7 N.D. -0.6 -0.6 N.D.
phage P. simiae OR Antrim MOI 0.1 -1.8 N.D. -0.4 -0.5 N.D.
phage P. simiae OR Antrim MOI 1 -1.8 N.D. -0.3 -0.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.2 N.D. -0.6 -0.6 N.D.
phage P. simiae Grant Run MOI 0.1 -1.8 N.D. -0.7 +0.2 N.D.
carbon source L-Valine (C) -2.2 N.D. -0.2 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 N.D. -0.3 -1.0 N.D.
phage P. simiae Grant Run MOI 10 -1.8 N.D. -0.3 +0.1 N.D.
phage P. simiae OR Antrim MOI 10 -1.4 N.D. -0.6 +0.0 N.D.
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.5 N.D. -0.0 -2.4 N.D.
soil soil sample 2; outgrowth in LB +0.1 N.D. -0.5 -1.5 N.D.
phage P. simiae Grant Run MOI 0.1 -2.0 N.D. -0.3 +0.5 N.D.
phage P. simiae Grant Run MOI 10 -1.5 N.D. -0.4 +0.2 N.D.
soil soil sample 3; outgrowth in LB +0.6 N.D. -0.3 -1.7 N.D.
stress Gentamicin 0.008 mg/ml -1.6 N.D. -0.4 +0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 N.D. +0.3 -1.1 N.D.
soil soil sample 6; outgrowth in LB +0.9 N.D. -0.2 +0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.5 N.D. +0.5 -0.3 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB +0.6 N.D. -0.0 +1.6 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +1.7 N.D. +0.0 +0.6 N.D.
carbon source Gly-Glu (C) +3.6 N.D. +0.1 -1.2 N.D.
carbon source Gly-Glu (C) +4.6 N.D. +0.5 -1.6 N.D.
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