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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_16930 and PS417_16935 overlap by 14 nucleotides
PS417_16935 and PS417_16940 are separated by 51 nucleotides
PS417_16940 and PS417_16945 are separated by 22 nucleotides
PS417_16945 and PS417_16950 are separated by 84 nucleotides
PS417_16930: PS417_16930 - histidine kinase, at 3,690,679 to 3,693,465
_16930
PS417_16935: PS417_16935 - diguanylate phosphodiesterase, at 3,693,452 to 3,694,639
_16935
PS417_16940: PS417_16940 - chemotaxis protein CheY, at 3,694,691 to 3,697,849
_16940
PS417_16945: PS417_16945 - LuxR family transcriptional regulator, at 3,697,872 to 3,698,516
_16945
PS417_16950: PS417_16950 - fimbrial protein, at 3,698,601 to 3,699,191
_16950
Group
Condition
PS417
_16930
PS417
_16935
PS417
_16940
PS417
_16945
PS417
_16950
phage
P. simiae OR1 MOI 0.1
-0.3
-0.8
+0.2
-0.3
-0.7
soil
soil sample 7; outgrowth in LB
+0.3
-1.0
+0.0
-0.8
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.3
+0.0
-0.6
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.2
+0.2
-1.6
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
-0.3
+0.4
-1.2
-0.6
soil
soil sample 4; outgrowth in LB
+0.2
-0.7
+0.2
-0.7
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.0
+0.0
-0.3
-1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.3
-0.4
+0.1
-0.7
+0.1
motility
inner cut, LB soft agar motility assay
-0.4
-0.5
-0.0
-0.6
+0.4
phage
P. simiae OR Antrim MOI 1
+0.3
-0.2
+0.2
+1.0
-2.4
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.2
-0.2
+0.1
-0.9
+0.4
phage
P. simiae Grant Run MOI 10
-0.1
-0.2
-0.3
+0.7
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
-0.3
+0.1
-0.9
+0.5
phage
P. simiae ORA MOI 0.1
+0.1
-0.3
-0.0
+0.8
-1.1
seeds
Growth on radish seeds for 72 hours
+0.0
-0.9
-0.2
+0.6
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.0
-0.0
-1.2
+0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.7
-0.5
+0.5
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.3
+0.5
-0.1
-0.6
+0.4
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
+0.0
+0.6
-0.1
+0.3
-0.8
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
-0.0
+0.3
-0.1
+0.8
-0.6
supernatant control
Vogels_fungal_media; pH 5.5
-0.3
-0.4
-0.1
+0.5
+0.8
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
-0.2
+0.3
-0.4
+0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
-0.4
+0.2
+0.2
+0.9
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.6
+0.4
+0.2
+0.9
-0.9
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
+0.6
+0.4
+0.7
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.1
+0.8
+0.8
+0.2
-0.5
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.1
-0.0
+0.2
-0.2
+1.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+1.1
+0.3
+0.2
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.8
+0.5
-0.1
+0.3
+0.2
seeds
Growth on radish seeds for 24 hours
+0.0
-0.1
-0.3
+0.7
+1.6
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