Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16870 and PS417_16875 are separated by 192 nucleotidesPS417_16875 and PS417_16880 are separated by 112 nucleotidesPS417_16880 and PS417_16885 are separated by 15 nucleotidesPS417_16885 and PS417_16890 are separated by 365 nucleotides PS417_16870: PS417_16870 - short-chain dehydrogenase, at 3,677,666 to 3,678,448 _16870 PS417_16875: PS417_16875 - signal peptidase, at 3,678,641 to 3,679,309 _16875 PS417_16880: PS417_16880 - deaminase/reductase, at 3,679,422 to 3,679,985 _16880 PS417_16885: PS417_16885 - hypothetical protein, at 3,680,001 to 3,680,699 _16885 PS417_16890: PS417_16890 - cysteinyl-tRNA synthetase, at 3,681,065 to 3,682,453 _16890
Group Condition PS417_16870 PS417_16875 PS417_16880 PS417_16885 PS417_16890
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.6 +0.2 +0.0 -1.4 N.D.
seeds Growth on radish seeds for 72 hours -0.2 -1.1 +0.3 -1.1 N.D.
phage P. simiae Grant Run MOI 0.1 -0.6 +0.2 -0.1 -1.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.5 -0.7 -0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 -0.2 -0.7 +0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.2 -0.3 -1.4 N.D.
carbon source Carbon source D-Mannitol 5 mM -0.7 +0.3 +0.1 -0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 -0.7 +0.1 -1.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -1.0 +0.0 +0.6 N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 -0.2 +0.0 -1.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 +0.4 -0.2 +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.0 +0.4 -1.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 -0.7 -0.3 +0.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 -0.4 -0.6 +0.4 N.D.
soil soil sample 4; outgrowth in LB +0.3 -1.0 -0.1 +0.8 N.D.
phage P. simiae OR Antrim MOI 1 -0.1 +0.7 +0.2 -0.8 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.5 -0.6 +0.8 N.D.
rhizosphere rhizosphere sample 2; outgrowth in LB -0.6 -0.1 -0.2 +1.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.6 -0.2 -0.5 +0.5 N.D.
phage P. simiae Grant Run MOI 10 +0.8 +0.6 +0.0 -0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 +0.4 -0.1 -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.3 +0.1 -0.2 -0.3 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.6 +0.8 +0.3 +0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 -0.4 +1.0 +0.6 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.9 -0.2 +0.1 N.D.
agar plate interaction control Taped volatile agar plate with no fungus +0.8 -0.5 +0.4 +0.7 N.D.
stress Choline chloride 300 mM +0.8 +0.4 +0.5 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.3 +0.4 +0.9 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.6 +0.1 +0.4 +0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 +0.2 +0.2 +0.9 N.D.
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