Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16845 and PS417_16850 overlap by 4 nucleotidesPS417_16850 and PS417_16855 overlap by 4 nucleotidesPS417_16855 and PS417_16860 are separated by 126 nucleotidesPS417_16860 and PS417_16865 are separated by 101 nucleotides PS417_16845: PS417_16845 - monovalent cation/H+ antiporter subunit E, at 3,675,555 to 3,676,043 _16845 PS417_16850: PS417_16850 - cation:proton antiporter, at 3,676,040 to 3,676,318 _16850 PS417_16855: PS417_16855 - cation:proton antiporter, at 3,676,315 to 3,676,653 _16855 PS417_16860: PS417_16860 - diadenosine tetraphosphate hydrolase, at 3,676,780 to 3,677,238 _16860 PS417_16865: PS417_16865 - HxlR family transcriptional regulator, at 3,677,340 to 3,677,669 _16865
Group Condition PS417_16845 PS417_16850 PS417_16855 PS417_16860 PS417_16865
stress L-Lysine 60 mM -4.5 -2.5 -1.7 +0.5 N.D.
stress Choline Lysine 25 mM -2.8 -3.1 -0.9 +0.1 N.D.
nophagecontrol Only library -0.1 -0.1 -1.3 -0.9 N.D.
seeds Growth on radish seeds for 72 hours -0.9 -0.5 -1.0 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.3 -1.3 -0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.8 -0.4 -2.4 -0.0 N.D.
supernatant control Vogels_fungal_media 0.1X +0.6 -0.1 -1.9 -0.4 N.D.
phage P. simiae OR Antrim MOI 1 +0.5 -0.2 -1.9 -0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.8 +0.3 -1.0 -0.2 N.D.
phage P. simiae Grant Run MOI 0.1 +0.3 -0.4 -1.6 +0.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 -0.9 -0.9 -0.1 N.D.
stress Doxycycline hyclate 0.001 mg/ml -0.5 +0.6 -1.4 -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.2 -1.4 +0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +0.7 -1.3 -0.1 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.5 -0.8 -1.1 -0.6 N.D.
soil soil sample 7; outgrowth in LB +0.5 -0.7 -0.8 +0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.3 +1.4 -1.6 -0.3 N.D.
soil soil sample 5; outgrowth in LB -0.0 -0.5 -1.0 +0.9 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.5 +0.8 -0.1 -0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.4 +1.0 +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.8 +0.4 -0.2 +1.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +1.0 -0.6 -0.3 N.D.
stress methylglyoxal 0.032 vol% -0.6 +0.5 +1.2 -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 +0.4 -1.2 +0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.9 +0.2 +1.8 -0.1 N.D.
carbon source L-Valine (C) -0.5 +0.7 +1.0 -0.2 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 +0.8 +1.0 -0.2 N.D.
root root sample 6; outgrowth in LB +1.0 -0.1 +0.0 +1.2 N.D.
seeds Growth on radish seeds for 72 hours +0.7 +0.9 +0.7 +0.2 N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.6 +1.1 +0.6 +0.7 N.D.
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