Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16650 and PS417_16655 are separated by 133 nucleotidesPS417_16655 and PS417_16660 are separated by 120 nucleotidesPS417_16660 and PS417_16665 are separated by 31 nucleotidesPS417_16665 and PS417_16675 are separated by 304 nucleotides PS417_16650: PS417_16650 - hypothetical protein, at 3,638,508 to 3,639,260 _16650 PS417_16655: PS417_16655 - N-hydroxyarylamine O-acetyltransferase, at 3,639,394 to 3,640,221 _16655 PS417_16660: PS417_16660 - hypothetical protein, at 3,640,342 to 3,640,578 _16660 PS417_16665: PS417_16665 - hypothetical protein, at 3,640,610 to 3,640,810 _16665 PS417_16675: PS417_16675 - hypothetical protein, at 3,641,115 to 3,641,636 _16675
Group Condition PS417_16650 PS417_16655 PS417_16660 PS417_16665 PS417_16675
phage JP1 MOI 10 +0.1 -0.1 N.D. -1.6 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.1 N.D. -1.4 -0.3
rhizosphere rhizosphere sample 2; outgrowth in LB +0.3 -0.1 N.D. -1.6 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.3 N.D. -0.7 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.5 -0.4 N.D. -0.4 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 -0.3 N.D. -0.2 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.1 N.D. -0.9 -0.1
phage P. simiae ORA MOI 0.1 +0.0 +0.4 N.D. -2.0 +0.2
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.3 -0.5 N.D. -0.7 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.4 N.D. -0.7 -0.2
soil soil sample 5; outgrowth in LB -0.2 +0.2 N.D. -1.3 +0.1
soil soil sample 2; outgrowth in LB +0.1 +0.1 N.D. -1.3 -0.1
no phage control no phage control -0.3 +0.2 N.D. -0.9 -0.2
phage JP1 MOI 1 +0.2 -0.2 N.D. -1.0 -0.1
soil soil sample 4; outgrowth in LB -0.3 +0.3 N.D. -0.8 -0.2
phage P. simiae OR Antrim MOI 1 -0.0 +0.1 N.D. -1.3 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.5 -0.1 N.D. -1.1 +0.0
stress methylglyoxal 0.032 vol% -0.1 +0.7 N.D. -0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.0 N.D. +0.4 -0.7
phage P. simiae OR Antrim MOI 1 +0.5 -0.3 N.D. -0.5 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.4 N.D. -0.6 -0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.4 -0.3 N.D. +0.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.4 N.D. -0.9 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.3 -0.4 N.D. +0.7 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.2 N.D. -0.3 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 +0.2 N.D. +1.1 -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.0 +0.4 N.D. +0.9 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.1 N.D. +0.8 +0.3
stress Gentamicin 0.008 mg/ml +0.2 +0.2 N.D. +1.0 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.2 N.D. +1.3 -0.1
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