Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16545 and PS417_16550 are separated by 125 nucleotidesPS417_16550 and PS417_16555 are separated by 83 nucleotidesPS417_16555 and PS417_16560 overlap by 8 nucleotidesPS417_16560 and PS417_16565 are separated by 231 nucleotides PS417_16545: PS417_16545 - DNA repair protein, at 3,615,389 to 3,616,012 _16545 PS417_16550: PS417_16550 - adenylosuccinate lyase, at 3,616,138 to 3,617,508 _16550 PS417_16555: PS417_16555 - cupin, at 3,617,592 to 3,618,758 _16555 PS417_16560: PS417_16560 - GNAT family acetyltransferase, at 3,618,751 to 3,619,173 _16560 PS417_16565: PS417_16565 - secretin, at 3,619,405 to 3,620,142 _16565
Group Condition PS417_16545 PS417_16550 PS417_16555 PS417_16560 PS417_16565
solid stress Fraxetin 3 mM; solid stress N.D. N.D. -2.7 -1.3 -1.1
solid stress Fraxetin 0.5 mM; solid stress N.D. N.D. -3.9 -0.6 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -3.5 -1.2 -0.1
solid stress Scopoletin 2 mM; solid stress N.D. N.D. -4.0 -0.4 -0.3
liquid stress Scopoletin 3 mM; liquid stress N.D. N.D. -3.6 -0.9 +0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -3.1 -0.6 -0.7
solid stress 80% Methanol 2 mM; solid stress N.D. N.D. -3.8 -0.3 -0.2
liquid stress 80% Methanol 2 mM; liquid stress N.D. N.D. -3.5 -0.5 -0.1
solid stress 80% Methanol 2 mM; solid stress N.D. N.D. -1.9 -1.9 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -4.6 +0.5 +0.1
solid stress 80% Methanol 3 mM; solid stress N.D. N.D. -3.9 -0.1 -0.0
liquid stress Scopoletin 2 mM; liquid stress N.D. N.D. -3.0 -0.8 -0.2
liquid stress 80% Methanol 3 mM; liquid stress N.D. N.D. -3.1 -1.0 +0.2
soil soil sample 4; outgrowth in LB N.D. N.D. -2.8 -0.6 -0.5
solid stress Scopoletin 2 mM; solid stress N.D. N.D. -2.9 -0.8 +0.1
agar plate interaction control Taped volatile agar plate with no fungus N.D. N.D. -2.3 -0.9 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.1 -1.1 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -4.1 +1.0 -0.4
phage P. simiae Grant Run MOI 0.1 N.D. N.D. -3.3 +0.5 -0.5
phage P. simiae OR1 MOI 0.1 N.D. N.D. -3.6 -0.0 +0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. N.D. -2.5 +0.5 -1.0
soil soil sample 2; outgrowth in LB N.D. N.D. -2.2 -1.6 +1.0
soil soil sample 7; outgrowth in LB N.D. N.D. -3.1 +0.5 -0.0
soil soil sample 3; outgrowth in LB N.D. N.D. -3.7 +0.7 +0.5
phage JP1 MOI 10 N.D. N.D. -2.0 +1.2 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +1.5 +0.9 -1.4
stress Neomycin 0.04 mg/ml N.D. N.D. +3.3 -0.5 -0.3
carbon source 2'-Deoxyinosine 5 mM (C) N.D. N.D. +2.1 +1.2 -0.7
stress Nalidixic 0.025 mg/ml N.D. N.D. +3.0 +0.6 +0.3
stress Gentamicin 0.008 mg/ml N.D. N.D. +7.0 +1.7 +1.7
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