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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_16545 and PS417_16550 are separated by 125 nucleotides
PS417_16550 and PS417_16555 are separated by 83 nucleotides
PS417_16555 and PS417_16560 overlap by 8 nucleotides
PS417_16560 and PS417_16565 are separated by 231 nucleotides
PS417_16545: PS417_16545 - DNA repair protein, at 3,615,389 to 3,616,012
_16545
PS417_16550: PS417_16550 - adenylosuccinate lyase, at 3,616,138 to 3,617,508
_16550
PS417_16555: PS417_16555 - cupin, at 3,617,592 to 3,618,758
_16555
PS417_16560: PS417_16560 - GNAT family acetyltransferase, at 3,618,751 to 3,619,173
_16560
PS417_16565: PS417_16565 - secretin, at 3,619,405 to 3,620,142
_16565
Group
Condition
PS417
_16545
PS417
_16550
PS417
_16555
PS417
_16560
PS417
_16565
solid stress
Fraxetin 3 mM; solid stress
N.D.
N.D.
-2.7
-1.3
-1.1
solid stress
Fraxetin 0.5 mM; solid stress
N.D.
N.D.
-3.9
-0.6
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
-3.5
-1.2
-0.1
solid stress
Scopoletin 2 mM; solid stress
N.D.
N.D.
-4.0
-0.4
-0.3
liquid stress
Scopoletin 3 mM; liquid stress
N.D.
N.D.
-3.6
-0.9
+0.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
-3.1
-0.6
-0.7
solid stress
80% Methanol 2 mM; solid stress
N.D.
N.D.
-3.8
-0.3
-0.2
liquid stress
80% Methanol 2 mM; liquid stress
N.D.
N.D.
-3.5
-0.5
-0.1
solid stress
80% Methanol 2 mM; solid stress
N.D.
N.D.
-1.9
-1.9
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
-4.6
+0.5
+0.1
solid stress
80% Methanol 3 mM; solid stress
N.D.
N.D.
-3.9
-0.1
-0.0
liquid stress
Scopoletin 2 mM; liquid stress
N.D.
N.D.
-3.0
-0.8
-0.2
liquid stress
80% Methanol 3 mM; liquid stress
N.D.
N.D.
-3.1
-1.0
+0.2
soil
soil sample 4; outgrowth in LB
N.D.
N.D.
-2.8
-0.6
-0.5
solid stress
Scopoletin 2 mM; solid stress
N.D.
N.D.
-2.9
-0.8
+0.1
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
N.D.
-2.3
-0.9
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-2.1
-1.1
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
-4.1
+1.0
-0.4
phage
P. simiae Grant Run MOI 0.1
N.D.
N.D.
-3.3
+0.5
-0.5
phage
P. simiae OR1 MOI 0.1
N.D.
N.D.
-3.6
-0.0
+0.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
N.D.
-2.5
+0.5
-1.0
soil
soil sample 2; outgrowth in LB
N.D.
N.D.
-2.2
-1.6
+1.0
soil
soil sample 7; outgrowth in LB
N.D.
N.D.
-3.1
+0.5
-0.0
soil
soil sample 3; outgrowth in LB
N.D.
N.D.
-3.7
+0.7
+0.5
phage
JP1 MOI 10
N.D.
N.D.
-2.0
+1.2
+0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
+1.5
+0.9
-1.4
stress
Neomycin 0.04 mg/ml
N.D.
N.D.
+3.3
-0.5
-0.3
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
N.D.
+2.1
+1.2
-0.7
stress
Nalidixic 0.025 mg/ml
N.D.
N.D.
+3.0
+0.6
+0.3
stress
Gentamicin 0.008 mg/ml
N.D.
N.D.
+7.0
+1.7
+1.7
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