Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16520 and PS417_16525 are separated by 81 nucleotidesPS417_16525 and PS417_16530 are separated by 493 nucleotidesPS417_16530 and PS417_16535 are separated by 226 nucleotidesPS417_16535 and PS417_16540 are separated by 59 nucleotides PS417_16520: PS417_16520 - cold-shock protein, at 3,609,200 to 3,609,478 _16520 PS417_16525: PS417_16525 - isocitrate dehydrogenase, at 3,609,560 to 3,610,816 _16525 PS417_16530: PS417_16530 - isocitrate dehydrogenase, at 3,611,310 to 3,613,535 _16530 PS417_16535: PS417_16535 - NUDIX hydrolase, at 3,613,762 to 3,614,208 _16535 PS417_16540: PS417_16540 - tRNA 2-thiouridylase, at 3,614,268 to 3,615,392 _16540
Group Condition PS417_16520 PS417_16525 PS417_16530 PS417_16535 PS417_16540
carbon source L-Valine (C) -1.0 -0.1 N.D. -2.1 N.D.
root root sample 6; outgrowth in LB -2.9 -0.3 N.D. +0.4 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB -1.5 -0.4 N.D. -0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.0 -0.4 N.D. -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 -0.2 N.D. -1.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.4 N.D. -1.4 N.D.
soil soil sample 2; outgrowth in LB -1.8 -0.4 N.D. -0.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.4 -0.1 N.D. +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.8 -0.3 N.D. -1.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.8 -0.2 N.D. -0.1 N.D.
carbon source octanoate (C) -0.8 -1.0 N.D. -0.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 -0.1 N.D. -0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -1.0 -0.4 N.D. -0.5 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.3 -0.1 N.D. -0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.3 +0.1 N.D. -0.6 N.D.
carbon source Uridine (C) -1.1 -0.2 N.D. -0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.8 -0.6 N.D. -0.4 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.2 -0.2 N.D. -0.3 N.D.
stress Gentamicin 0.008 mg/ml -0.7 +0.2 N.D. -1.1 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.7 +0.0 N.D. +0.1 N.D.
stress Nalidixic 0.025 mg/ml -1.5 -0.1 N.D. +0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.0 +0.1 N.D. +0.6 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 -0.2 N.D. +0.8 N.D.
seeds Growth on radish seeds for 72 hours +0.8 +0.0 N.D. -1.0 N.D.
phage P. simiae ORA MOI 10 -0.8 +0.2 N.D. +1.1 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.1 N.D. +1.5 N.D.
stress Doxycycline hyclate 0.001 mg/ml +1.5 +0.0 N.D. -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 +0.4 N.D. +1.5 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +1.4 -0.0 N.D. +0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.1 +0.4 N.D. +0.9 N.D.
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