Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16395 and PS417_16400 overlap by 4 nucleotidesPS417_16400 and PS417_16405 overlap by 17 nucleotidesPS417_16405 and PS417_16410 are separated by 88 nucleotidesPS417_16410 and PS417_16415 are separated by 67 nucleotides PS417_16395: PS417_16395 - arsenate reductase, at 3,586,817 to 3,587,170 _16395 PS417_16400: PS417_16400 - NAD(P)H-quinone oxidoreductase, at 3,587,167 to 3,587,781 _16400 PS417_16405: PS417_16405 - membrane protein, at 3,587,765 to 3,588,181 _16405 PS417_16410: PS417_16410 - 3-methyladenine DNA glycosylase, at 3,588,270 to 3,588,941 _16410 PS417_16415: PS417_16415 - tRNA 2-thiocytidine biosynthesis protein TtcA, at 3,589,009 to 3,589,833 _16415
Group Condition PS417_16395 PS417_16400 PS417_16405 PS417_16410 PS417_16415
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 -6.5 -0.3 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -2.0 -0.0 N.D. -1.0
carbon source 2'-Deoxyinosine 5 mM (C) -1.1 -0.7 -0.4 N.D. -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.3 -1.3 +0.1 N.D. -0.5
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.4 -1.4 -0.2 N.D. +0.2
phage P. simiae OR Antrim MOI 0.1 -0.2 -1.3 -0.4 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.2 -0.3 N.D. -1.2
rhizosphere rhizosphere sample 2; outgrowth in LB -0.6 -1.0 -0.1 N.D. +0.2
seeds Growth on radish seeds for 72 hours +1.0 -0.9 -0.6 N.D. -0.7
no phage control no phage control -0.2 -1.4 +0.1 N.D. +0.4
phage Wcs_1 +1.0 -2.2 -0.2 N.D. +0.3
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.5 -0.9 -0.2 N.D. -0.3
stress Cisplatin 0.01 mg/ml -0.4 +0.7 +0.4 N.D. -1.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +1.3 +0.0 N.D. -1.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 +0.8 +0.1 N.D. -0.9
phage JP1 MOI 10 -1.4 +0.5 -0.0 N.D. +0.5
phage P. simiae OR1 MOI 0.1 -0.5 -1.0 +0.3 N.D. +0.9
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 +0.9 -0.2 N.D. -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 +0.4 -0.6 N.D. -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.5 +0.7 -0.4 N.D. -0.5
phage JP1 MOI 10 +1.2 -0.2 +0.0 N.D. -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +1.0 +0.4 N.D. -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.3 +1.0 +0.2 N.D. -0.7
phage P. simiae Grant Run MOI 10 -0.5 +1.1 +0.0 N.D. +0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 +1.5 +0.1 N.D. -0.3
carbon source L-Valine (C) +1.5 -0.1 -0.0 N.D. -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.4 +0.3 N.D. +1.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.0 +0.5 +0.1 N.D. +0.4
seeds Growth on radish seeds for 72 hours -0.0 +1.5 -0.0 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.5 -0.3 -0.1 N.D. +1.9
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