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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_16390 and PS417_16395 are separated by 115 nucleotides
PS417_16395 and PS417_16400 overlap by 4 nucleotides
PS417_16400 and PS417_16405 overlap by 17 nucleotides
PS417_16405 and PS417_16410 are separated by 88 nucleotides
PS417_16390: PS417_16390 - peroxiredoxin, at 3,586,240 to 3,586,701
_16390
PS417_16395: PS417_16395 - arsenate reductase, at 3,586,817 to 3,587,170
_16395
PS417_16400: PS417_16400 - NAD(P)H-quinone oxidoreductase, at 3,587,167 to 3,587,781
_16400
PS417_16405: PS417_16405 - membrane protein, at 3,587,765 to 3,588,181
_16405
PS417_16410: PS417_16410 - 3-methyladenine DNA glycosylase, at 3,588,270 to 3,588,941
_16410
Group
Condition
PS417
_16390
PS417
_16395
PS417
_16400
PS417
_16405
PS417
_16410
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
+0.0
-6.5
-0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.4
-2.0
-0.0
N.D.
carbon source
2'-Deoxyinosine 5 mM (C)
+0.0
-1.1
-0.7
-0.4
N.D.
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
-0.2
-0.4
-1.4
-0.2
N.D.
phage
Wcs_0.1
-0.2
-1.0
-0.4
-0.2
N.D.
phage
P. simiae OR1 MOI 0.1
-0.5
-0.5
-1.0
+0.3
N.D.
phage
Wcs_1
-0.1
+1.0
-2.2
-0.2
N.D.
phage
P. simiae OR Antrim MOI 0.1
+0.6
-0.2
-1.3
-0.4
N.D.
phage
JP1 MOI 10
-0.3
-1.4
+0.5
-0.0
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
+0.5
-1.0
-0.2
N.D.
seeds
Growth on radish seeds for 72 hours
-0.5
+1.0
-0.9
-0.6
N.D.
stress
Neomycin 0.04 mg/ml
-0.9
+0.6
-0.6
-0.2
N.D.
solid stress
Fraxetin 3 mM; solid stress
-1.3
+0.5
+0.4
-0.1
N.D.
motility
inner cut, LB soft agar motility assay
+0.7
-0.7
-0.4
-0.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
+0.4
-1.0
-0.0
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.8
+0.8
+0.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.7
+0.4
-0.6
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.3
-0.6
+0.8
+0.2
N.D.
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
-0.6
-0.2
+1.5
+0.1
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.3
+1.5
-0.3
-0.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.5
+1.0
+0.4
N.D.
phage
P. simiae Grant Run MOI 10
+0.4
-0.5
+1.1
+0.0
N.D.
stress
methylglyoxal 0.032 vol%
+0.5
+0.5
+0.6
-0.4
N.D.
phage
P. simiae ORA MOI 10
-0.3
+0.6
+1.0
-0.1
N.D.
stress
Gentamicin 0.008 mg/ml
+2.3
-0.0
-1.0
+0.2
N.D.
stress
Paraquat dichloride 0.008 mg/ml
+2.3
-0.1
-0.4
-0.1
N.D.
carbon source
L-Valine (C)
+0.3
+1.5
-0.1
-0.0
N.D.
seeds
Growth on radish seeds for 72 hours
+0.4
-0.0
+1.5
-0.0
N.D.
phage
P. simiae Grant Run MOI 0.1
+0.4
+0.5
+1.0
+0.2
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.6
+1.0
+0.5
+0.1
N.D.
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