Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16345 and PS417_16350 are separated by 73 nucleotidesPS417_16350 and PS417_16355 are separated by 147 nucleotidesPS417_16355 and PS417_16360 overlap by 4 nucleotidesPS417_16360 and PS417_16365 overlap by 14 nucleotides PS417_16345: PS417_16345 - biopolymer transporter ExbB, at 3,575,449 to 3,576,084 _16345 PS417_16350: PS417_16350 - competence protein ComEC, at 3,576,158 to 3,578,371 _16350 PS417_16355: PS417_16355 - hypothetical protein, at 3,578,519 to 3,579,034 _16355 PS417_16360: PS417_16360 - histidine kinase, at 3,579,031 to 3,580,401 _16360 PS417_16365: PS417_16365 - transcriptional regulator, at 3,580,388 to 3,581,116 _16365
Group Condition PS417_16345 PS417_16350 PS417_16355 PS417_16360 PS417_16365
liquid stress Fraxetin 3 mM; liquid stress N.D. -0.3 -0.1 -2.2 N.D.
liquid stress Fraxetin 3 mM; liquid stress N.D. -0.1 +0.0 -1.9 N.D.
solid stress Fraxetin 3 mM; solid stress N.D. -0.3 -0.1 -1.4 N.D.
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. -0.7 -0.6 -0.4 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -0.8 -0.1 -0.7 N.D.
liquid stress Fraxetin 3 mM; liquid stress N.D. +0.1 +0.0 -1.4 N.D.
solid stress Fraxetin 2 mM; solid stress N.D. +0.2 -0.0 -1.5 N.D.
solid stress Fraxetin 3 mM; solid stress N.D. -0.1 -0.1 -1.1 N.D.
carbon source octanoate (C) N.D. -0.4 -0.3 -0.6 N.D.
liquid stress Fraxetin 3 mM; liquid stress N.D. -0.0 +0.1 -1.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.1 +0.1 -0.2 N.D.
liquid stress Fraxetin 3 mM; liquid stress N.D. +0.1 +0.1 -1.3 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.6 -0.3 -0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 +0.0 -1.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.5 +0.1 -0.6 N.D.
solid stress Fraxetin 1 mM; solid stress N.D. +0.1 -0.1 -0.9 N.D.
carbon source Carbon source L-Ornithine 10 mM N.D. +0.1 -0.3 -0.8 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.0 -0.3 +0.5 N.D.
solid stress Fraxetin 3 mM; solid stress N.D. +0.3 +0.1 -1.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.6 +0.7 -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 +0.4 -0.3 N.D.
phage Wcs_1 N.D. -0.8 +0.1 +0.5 N.D.
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.6 -0.1 +0.6 N.D.
stress Doxycycline hyclate 0.001 mg/ml N.D. -0.1 -0.4 +0.8 N.D.
soil soil sample 2; outgrowth in LB N.D. -0.5 +0.2 +0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.3 -0.0 +1.1 N.D.
stress Neomycin 0.04 mg/ml N.D. +0.4 -0.2 +0.7 N.D.
root root sample 6; outgrowth in LB N.D. +0.6 -0.2 +0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.7 +0.2 +0.4 N.D.
phage P. simiae OR Antrim MOI 0.1 N.D. +0.6 +0.6 +0.5 N.D.
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