Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16305 and PS417_16310 are separated by 152 nucleotidesPS417_16310 and PS417_16315 are separated by 62 nucleotidesPS417_16315 and PS417_16320 overlap by 4 nucleotidesPS417_16320 and PS417_16325 overlap by 1 nucleotides PS417_16305: PS417_16305 - tRNA-Ser, at 3,570,586 to 3,570,676 _16305 PS417_16310: PS417_16310 - membrane protein, at 3,570,829 to 3,571,500 _16310 PS417_16315: PS417_16315 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 3,571,563 to 3,572,582 _16315 PS417_16320: PS417_16320 - protein tyrosine phosphatase, at 3,572,579 to 3,573,043 _16320 PS417_16325: PS417_16325 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 3,573,043 to 3,573,807 _16325
Group Condition PS417_16305 PS417_16310 PS417_16315 PS417_16320 PS417_16325
solid stress Fraxetin 3 mM; solid stress N.D. -2.3 N.D. -1.2 N.D.
stress Gentamicin 0.008 mg/ml N.D. -2.1 N.D. -0.4 N.D.
no stress control on plate growth on LB_noSalt plate N.D. -1.9 N.D. -0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -2.2 N.D. +0.2 N.D.
no stress control on plate growth on LB_noSalt plate N.D. -1.8 N.D. -0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.8 N.D. -0.7 N.D.
supernatant control Vogels_fungal_media 0.1X N.D. -1.0 N.D. -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X N.D. -1.1 N.D. -0.4 N.D.
soil soil sample 6; outgrowth in LB N.D. -1.5 N.D. +0.0 N.D.
solid stress Fraxetin 3 mM; solid stress N.D. -1.2 N.D. -0.2 N.D.
soil soil sample 3; outgrowth in LB N.D. -1.1 N.D. -0.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -1.1 N.D. -0.3 N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.0 N.D. -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.4 N.D. -1.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.2 N.D. +0.2 N.D.
solid stress Fraxetin 3 mM; solid stress N.D. -1.2 N.D. +0.3 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.4 N.D. +0.5 N.D.
phage JP1 MOI 10 N.D. -1.1 N.D. +0.6 N.D.
seeds Growth on radish seeds for 72 hours N.D. -1.0 N.D. +0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 N.D. +0.7 N.D.
liquid stress Fraxetin 2 mM; liquid stress N.D. +0.8 N.D. -0.8 N.D.
supernatant control Vogels_fungal_media 0.1X N.D. -0.9 N.D. +1.0 N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.8 N.D. +0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 N.D. +1.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 N.D. +1.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.5 N.D. +1.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.9 N.D. +2.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +1.1 N.D. +0.5 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 N.D. +1.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +1.4 N.D. +0.9 N.D.
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