Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16250 and PS417_16255 overlap by 4 nucleotidesPS417_16255 and PS417_16260 are separated by 76 nucleotidesPS417_16260 and PS417_16265 are separated by 194 nucleotidesPS417_16265 and PS417_16270 are separated by 134 nucleotides PS417_16250: PS417_16250 - iron dicitrate transport regulator FecR, at 3,561,551 to 3,562,483 _16250 PS417_16255: PS417_16255 - RNA polymerase sigma factor, at 3,562,480 to 3,563,022 _16255 PS417_16260: PS417_16260 - TonB-dependent receptor, at 3,563,099 to 3,565,216 _16260 PS417_16265: PS417_16265 - hypothetical protein, at 3,565,411 to 3,565,680 _16265 PS417_16270: PS417_16270 - hypothetical protein, at 3,565,815 to 3,566,564 _16270
Group Condition PS417_16250 PS417_16255 PS417_16260 PS417_16265 PS417_16270
carbon source L-Valine (C) N.D. -1.3 +0.1 -0.6 -0.1
phage JP1 MOI 0.1 N.D. -0.6 +0.1 -0.4 -0.6
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.8 -0.1 -0.4 -0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.3 +0.3 -0.4 -0.6
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. -1.1 -0.1 +0.0 +0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -0.9 -0.1 +0.4 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 -0.1 -0.5 -0.8
phage Wcs_1 N.D. +0.6 +0.3 -0.6 -0.9
phage P. simiae OR Antrim MOI 1 N.D. -1.2 +0.2 +0.6 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 -0.1 -0.8 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.7 +0.1 -0.3 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.6 +0.1 -0.6 -0.2
stress Gentamicin 0.008 mg/ml N.D. +0.2 -0.0 -0.8 +0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.4 +0.3 -0.5 +0.3
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. -0.6 +0.4 -0.1 +1.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 +0.5 -0.3 +0.6
rhizosphere rhizosphere sample 8; outgrowth in LB N.D. -0.3 +0.5 -0.4 +1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.4 +0.2 +0.6 +0.7
soil soil sample 8; outgrowth in LB N.D. +0.3 +0.3 -0.4 +0.9
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 -0.1 -0.2 +1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 +0.1 +0.5 +0.9
root root sample 6; outgrowth in LB N.D. -0.3 +0.8 -0.2 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +1.1 +0.0 -0.0 +0.3
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x N.D. +1.2 +0.2 -0.0 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.4 +0.4 +0.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +1.5 +0.0 +0.0 +0.1
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.8 +0.7 +0.0 +0.3
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. +0.6 +0.6 -0.0 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +1.7 +0.0 -0.2 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.9 +0.1 +0.1 +1.1
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