Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16205 and PS417_16210 are separated by 48 nucleotidesPS417_16210 and PS417_16215 are separated by 75 nucleotidesPS417_16215 and PS417_16220 are separated by 32 nucleotidesPS417_16220 and PS417_16225 are separated by 10 nucleotides PS417_16205: PS417_16205 - hypothetical protein, at 3,551,980 to 3,552,411 _16205 PS417_16210: PS417_16210 - hypothetical protein, at 3,552,460 to 3,553,473 _16210 PS417_16215: PS417_16215 - ABC transporter substrate-binding protein, at 3,553,549 to 3,555,291 _16215 PS417_16220: PS417_16220 - membrane protein, at 3,555,324 to 3,555,596 _16220 PS417_16225: PS417_16225 - sugar ABC transporter permease, at 3,555,607 to 3,556,407 _16225
Group Condition PS417_16205 PS417_16210 PS417_16215 PS417_16220 PS417_16225
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.4 +0.0 -0.1 N.D. -0.4
stress methylglyoxal 0.032 vol% -0.7 -0.3 +0.1 N.D. -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 -0.4 -0.3 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.6 -0.1 N.D. -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.3 -0.5 +0.2 N.D. -0.4
phage JP1 MOI 1 -0.4 +0.4 -0.3 N.D. -0.5
phage P. simiae Grant Run MOI 10 -0.6 +0.1 +0.3 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -0.3 -0.2 N.D. -0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.3 -0.1 N.D. +0.3
no phage control Time6-nophage +0.7 -0.2 -0.6 N.D. +0.0
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.1 +0.1 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.5 +0.2 +0.3 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.2 -0.5 N.D. +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.7 -0.1 -0.1 N.D. -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 -0.4 -0.0 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +0.5 -0.5 N.D. +0.3
motility inner cut, LB soft agar motility assay -0.4 +0.2 +0.3 N.D. +0.3
no phage control no phage control +0.6 +0.3 -0.0 N.D. -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.3 -0.4 N.D. +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 -0.2 -0.2 N.D. +0.4
phage P. simiae OR Antrim MOI 0.1 +0.4 +0.3 -0.2 N.D. +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.5 +0.1 +0.2 N.D. -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.2 -0.2 N.D. +0.4
supernatant control Vogels_fungal_media; pH 5.5 +0.3 +0.3 -0.2 N.D. +0.4
seeds Growth on radish seeds for 72 hours +0.6 +0.2 +0.3 N.D. -0.1
carbon source 2'-Deoxyinosine 5 mM (C) +0.3 +0.4 +0.5 N.D. +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 +0.1 +0.3 N.D. +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB +0.6 +0.3 +0.2 N.D. +0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +1.4 +0.1 +0.0 N.D. +0.2
seeds Growth on radish seeds for 72 hours +1.2 +0.2 +0.4 N.D. +0.5
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