Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01600 and PS417_01605 are separated by 259 nucleotidesPS417_01605 and PS417_01610 are separated by 151 nucleotidesPS417_01610 and PS417_01615 are separated by 1 nucleotidesPS417_01615 and PS417_01620 are separated by 41 nucleotides PS417_01600: PS417_01600 - peptidase M23, at 352,199 to 353,494 _01600 PS417_01605: PS417_01605 - phosphoglycerate mutase, at 353,754 to 355,280 _01605 PS417_01610: PS417_01610 - sulfurtransferase, at 355,432 to 355,845 _01610 PS417_01615: PS417_01615 - glutaredoxin, at 355,847 to 356,101 _01615 PS417_01620: PS417_01620 - preprotein translocase subunit SecB, at 356,143 to 356,622 _01620
Group Condition PS417_01600 PS417_01605 PS417_01610 PS417_01615 PS417_01620
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -0.9 -3.6 N.D. N.D. -4.5
soil soil sample 5; outgrowth in LB -0.3 -2.8 N.D. N.D. -3.5
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x -0.6 -4.7 N.D. N.D. -1.3
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.9 -2.7 N.D. N.D. -3.0
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.5 -4.4 N.D. N.D. -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 -3.3 N.D. N.D. -1.7
exudates RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 1x -0.2 -4.8 N.D. N.D. -0.8
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x -0.3 -4.8 N.D. N.D. -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.7 -2.9 N.D. N.D. -3.4
soil soil sample 3; outgrowth in LB -0.6 -2.7 N.D. N.D. -2.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.1 -4.0 N.D. N.D. -1.5
carbon source L-Valine (C) -1.2 -4.0 N.D. N.D. -0.3
stress Choline Lysine 25 mM -0.8 -4.3 N.D. N.D. -0.4
carbon source RCH2_defined_glucose with MS_media 1x -0.3 -4.9 N.D. N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -3.4 N.D. N.D. -1.9
exudates RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 1x -0.3 -4.1 N.D. N.D. -1.0
stress L-Lysine 60 mM -1.5 -3.3 N.D. N.D. -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -3.3 N.D. N.D. -1.7
carbon source D-Mannose (C) -0.0 -4.7 N.D. N.D. -0.4
carbon source pyruvate (C) -0.3 -4.3 N.D. N.D. -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.8 -3.0 N.D. N.D. -1.2
carbon source Putrescine (C) +0.1 -4.4 N.D. N.D. -0.5
carbon source D,L-Lactate (C) +0.1 -4.7 N.D. N.D. -0.3
carbon source D-Alanine (C) +0.2 -4.7 N.D. N.D. -0.3
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.2 -3.9 N.D. N.D. -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -3.7 N.D. N.D. -1.1
carbon source D-Glucose-6-Phosphate (C) +0.3 -3.6 N.D. N.D. +2.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +3.5 -1.2 N.D. N.D. -2.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +3.2 -1.4 N.D. N.D. -1.5
carbon source D-Glucose-6-Phosphate (C) -0.1 -3.3 N.D. N.D. +4.5
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