Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_16085 and PS417_16090 are separated by 199 nucleotidesPS417_16090 and PS417_16095 are separated by 29 nucleotidesPS417_16095 and PS417_16100 overlap by 4 nucleotidesPS417_16100 and PS417_16105 are separated by 422 nucleotides PS417_16085: PS417_16085 - LysR family transcriptional regulator, at 3,526,400 to 3,527,281 _16085 PS417_16090: PS417_16090 - hypothetical protein, at 3,527,481 to 3,528,098 _16090 PS417_16095: PS417_16095 - amidohydrolase, at 3,528,128 to 3,529,966 _16095 PS417_16100: PS417_16100 - antibiotic biosynthesis monooxygenase, at 3,529,963 to 3,530,553 _16100 PS417_16105: PS417_16105 - LysR family transcriptional regulator, at 3,530,976 to 3,531,884 _16105
Group Condition PS417_16085 PS417_16090 PS417_16095 PS417_16100 PS417_16105
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 N.D. -1.7 +0.1 -0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 N.D. -0.9 -0.5 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 N.D. -0.3 -0.8 -0.3
liquid stress Fraxetin 2 mM; liquid stress +0.1 N.D. -0.5 -0.5 -0.7
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) -0.8 N.D. -0.3 -0.5 +0.1
carbon source L-Valine (C) +0.5 N.D. -1.0 +0.2 -0.9
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. -0.2 -0.7 -0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.0 N.D. -0.2 +0.5 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. -0.7 +0.4 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -0.7 +0.5 -0.5
soil soil sample 5; outgrowth in LB -0.8 N.D. +0.4 +0.4 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 N.D. +0.8 -1.5 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.8 N.D. +0.6 -0.3 +0.1
phage JP1 MOI 10 -0.6 N.D. +0.2 +0.6 -0.6
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.6 N.D. +0.2 -0.8 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 N.D. +0.5 +0.4 -1.1
motility inner cut, LB soft agar motility assay -0.2 N.D. +0.3 +0.6 -0.8
carbon source L-Leucine (C) +0.5 N.D. -0.1 -0.8 +0.4
phage JP1 MOI 1 +0.8 N.D. -0.3 +0.5 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 N.D. -0.5 +0.3 -0.3
seeds Growth on radish seeds for 72 hours +1.5 N.D. -0.4 +0.3 -1.3
phage Wcs_0.1 +0.2 N.D. -0.6 +0.9 -0.3
phage P. simiae OR1 MOI 0.1 +0.3 N.D. -0.0 +0.8 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.9 N.D. +0.5 -0.3 -0.3
phage P. simiae OR Antrim MOI 0.1 +0.6 N.D. -0.4 +0.2 +0.6
phage JP1 MOI 0.1 +0.3 N.D. -0.1 +1.4 -0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.6 N.D. +0.3 +0.8 +0.0
phage P. simiae ORA MOI 0.1 +0.4 N.D. +0.5 +0.2 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 N.D. +1.1 +0.4 +0.2
stress methylglyoxal 0.032 vol% +0.9 N.D. +0.1 +1.0 +0.8
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