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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_16085 and PS417_16090 are separated by 199 nucleotides
PS417_16090 and PS417_16095 are separated by 29 nucleotides
PS417_16095 and PS417_16100 overlap by 4 nucleotides
PS417_16100 and PS417_16105 are separated by 422 nucleotides
PS417_16085: PS417_16085 - LysR family transcriptional regulator, at 3,526,400 to 3,527,281
_16085
PS417_16090: PS417_16090 - hypothetical protein, at 3,527,481 to 3,528,098
_16090
PS417_16095: PS417_16095 - amidohydrolase, at 3,528,128 to 3,529,966
_16095
PS417_16100: PS417_16100 - antibiotic biosynthesis monooxygenase, at 3,529,963 to 3,530,553
_16100
PS417_16105: PS417_16105 - LysR family transcriptional regulator, at 3,530,976 to 3,531,884
_16105
Group
Condition
PS417
_16085
PS417
_16090
PS417
_16095
PS417
_16100
PS417
_16105
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
N.D.
-1.7
+0.1
-0.9
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
N.D.
-0.9
-0.5
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
N.D.
-0.3
-0.8
-0.3
liquid stress
Fraxetin 2 mM; liquid stress
+0.1
N.D.
-0.5
-0.5
-0.7
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
-0.8
N.D.
-0.3
-0.5
+0.1
carbon source
L-Valine (C)
+0.5
N.D.
-1.0
+0.2
-0.9
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.3
N.D.
-0.2
-0.7
-0.5
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.0
N.D.
-0.2
+0.5
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
N.D.
-0.7
+0.4
-0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.1
N.D.
-0.7
+0.5
-0.5
soil
soil sample 5; outgrowth in LB
-0.8
N.D.
+0.4
+0.4
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
N.D.
+0.8
-1.5
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.8
N.D.
+0.6
-0.3
+0.1
phage
JP1 MOI 10
-0.6
N.D.
+0.2
+0.6
-0.6
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
+0.6
N.D.
+0.2
-0.8
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
N.D.
+0.5
+0.4
-1.1
motility
inner cut, LB soft agar motility assay
-0.2
N.D.
+0.3
+0.6
-0.8
carbon source
L-Leucine (C)
+0.5
N.D.
-0.1
-0.8
+0.4
phage
JP1 MOI 1
+0.8
N.D.
-0.3
+0.5
-0.9
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.7
N.D.
-0.5
+0.3
-0.3
seeds
Growth on radish seeds for 72 hours
+1.5
N.D.
-0.4
+0.3
-1.3
phage
Wcs_0.1
+0.2
N.D.
-0.6
+0.9
-0.3
phage
P. simiae OR1 MOI 0.1
+0.3
N.D.
-0.0
+0.8
-0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.9
N.D.
+0.5
-0.3
-0.3
phage
P. simiae OR Antrim MOI 0.1
+0.6
N.D.
-0.4
+0.2
+0.6
phage
JP1 MOI 0.1
+0.3
N.D.
-0.1
+1.4
-0.3
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.6
N.D.
+0.3
+0.8
+0.0
phage
P. simiae ORA MOI 0.1
+0.4
N.D.
+0.5
+0.2
+1.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.5
N.D.
+1.1
+0.4
+0.2
stress
methylglyoxal 0.032 vol%
+0.9
N.D.
+0.1
+1.0
+0.8
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