Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15935 and PS417_15940 are separated by 4 nucleotidesPS417_15940 and PS417_15945 are separated by 177 nucleotidesPS417_15945 and PS417_15950 are separated by 76 nucleotidesPS417_15950 and PS417_15955 are separated by 99 nucleotides PS417_15935: PS417_15935 - Rrf2 family transcriptional regulator, at 3,486,916 to 3,487,353 _15935 PS417_15940: PS417_15940 - mechanosensitive ion channel protein MscS, at 3,487,358 to 3,488,803 _15940 PS417_15945: PS417_15945 - quercetin 2,3-dioxygenase, at 3,488,981 to 3,489,847 _15945 PS417_15950: PS417_15950 - major facilitator transporter, at 3,489,924 to 3,491,129 _15950 PS417_15955: PS417_15955 - secretion protein HylD, at 3,491,229 to 3,492,335 _15955
Group Condition PS417_15935 PS417_15940 PS417_15945 PS417_15950 PS417_15955
rhizosphere rhizosphere sample 2; outgrowth in LB -2.5 -0.6 N.D. -0.1 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -3.5 +0.3 N.D. -0.2 +0.3
carbon source L-Valine (C) -3.9 +0.5 N.D. -0.1 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.8 -0.1 N.D. +0.2 -0.2
carbon source L-Valine (C) -2.8 -0.0 N.D. +0.4 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.5 +0.1 N.D. +0.5 +0.2
phage P. simiae OR Antrim MOI 10 -1.6 -0.1 N.D. -0.0 +0.1
carbon source L-Leucine (C) -1.4 -0.2 N.D. +0.1 +0.3
nitrogen source Inosine (N) -1.0 +0.3 N.D. +0.1 -0.5
agar plate interaction control Taped volatile agar plate with no fungus -1.2 -0.3 N.D. -0.1 +0.5
carbon source 2'-Deoxyinosine 5 mM (C) +0.5 -0.9 N.D. -0.3 -0.4
phage JP1 MOI 0.1 +0.5 -0.7 N.D. -0.1 -0.8
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.0 -0.0 N.D. -0.4 +0.4
stress methylglyoxal 0.032 vol% +0.2 -0.3 N.D. +0.3 -1.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.7 +0.1 N.D. +0.0 +0.9
soil soil sample 3; outgrowth in LB -0.8 -0.5 N.D. +0.1 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.1 +0.2 N.D. -0.1 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.6 N.D. +0.8 -0.1
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.7 +0.1 N.D. -0.2 -0.7
root root sample 6; outgrowth in LB +0.9 -0.6 N.D. -0.0 -0.4
nophagecontrol Only library +1.1 -0.1 N.D. -0.4 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +1.0 N.D. -0.1 -0.3
nophagecontrol TIme6-nophage +1.4 +0.1 N.D. +0.3 -0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.9 -0.3 N.D. +0.3 -0.7
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.4 N.D. +0.2 +1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 +0.1 N.D. +0.9 -0.5
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 +0.0 N.D. +0.3 +1.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml +1.7 +0.4 N.D. -0.4 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +1.0 +0.2 N.D. -0.1 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.4 +0.4 N.D. -0.0 +0.2
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