Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15615 and PS417_15620 are separated by 163 nucleotidesPS417_15620 and PS417_15625 are separated by 191 nucleotidesPS417_15625 and PS417_15630 are separated by 121 nucleotidesPS417_15630 and PS417_15635 overlap by 36 nucleotides PS417_15615: PS417_15615 - short-chain dehydrogenase, at 3,426,506 to 3,427,363 _15615 PS417_15620: PS417_15620 - alpha/beta hydrolase, at 3,427,527 to 3,428,453 _15620 PS417_15625: PS417_15625 - lactate dehydrogenase, at 3,428,645 to 3,429,805 _15625 PS417_15630: PS417_15630 - DeoR faimly transcriptional regulator, at 3,429,927 to 3,430,712 _15630 PS417_15635: PS417_15635 - hypothetical protein, at 3,430,677 to 3,431,414 _15635
Group Condition PS417_15615 PS417_15620 PS417_15625 PS417_15630 PS417_15635
phage JP1 MOI 10 -0.5 +0.0 -0.4 -0.5 -0.6
agar plate interaction control Taped volatile agar plate with no fungus +0.1 -1.2 -0.6 -0.2 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 -0.3 -0.3 +0.1 -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.3 -0.6 -0.2 +0.2
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) +0.0 -0.1 +0.2 -0.6 -0.6
root root sample 6; outgrowth in LB +0.1 -0.2 +0.1 -0.3 -0.8
carbon source L-Valine (C) -0.6 +0.2 -0.1 -0.3 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 -0.2 -0.5 -0.3 -0.2
rhizosphere rhizosphere sample 2; outgrowth in LB +0.2 -1.0 +0.3 -0.2 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.3 +0.4 -0.4 -0.3 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.6 +0.5 +0.2 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.4 -0.2 -0.0 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.1 -0.3 +0.2 -0.7
carbon source 2'-Deoxyinosine 5 mM (C) +0.3 +0.4 +0.3 -0.6 -0.7
phage JP1 MOI 0.1 +0.3 +0.3 -0.3 -0.4 -0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 +0.3 -0.4 +0.4 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.5 +0.7 -0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.3 +0.8 -0.6 -0.4
phage P. simiae OR Antrim MOI 1 +0.1 +0.0 -0.2 +0.5 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.6 +0.1 -0.2 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.2 +0.6 +0.1 -0.3
stress Gentamicin 0.008 mg/ml -0.1 +0.5 -0.5 +0.1 +0.5
soil soil sample 3; outgrowth in LB -0.2 +0.4 +0.4 +0.5 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 -0.3 +0.3 +0.5 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 +0.3 +0.4 +0.4 +0.1
phage P. simiae Grant Run MOI 10 +0.2 +0.5 +0.5 -0.2 +0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 +0.2 +0.4 +0.4 +0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.0 +0.5 +0.5 -0.2 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.1 +0.1 -0.0 +1.5
seeds Growth on radish seeds for 72 hours +0.4 +0.0 -0.2 +0.9 +0.3
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