Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15595 and PS417_15605 are separated by 4993 nucleotidesPS417_15605 and PS417_15610 are separated by 196 nucleotidesPS417_15610 and PS417_15615 are separated by 11 nucleotidesPS417_15615 and PS417_15620 are separated by 163 nucleotides PS417_15595: PS417_15595 - LexA family transcriptional regulator, at 3,419,710 to 3,420,327 _15595 PS417_15605: PS417_15605 - hypothetical protein, at 3,425,321 to 3,425,710 _15605 PS417_15610: PS417_15610 - GDP-mannose pyrophosphatase, at 3,425,907 to 3,426,494 _15610 PS417_15615: PS417_15615 - short-chain dehydrogenase, at 3,426,506 to 3,427,363 _15615 PS417_15620: PS417_15620 - alpha/beta hydrolase, at 3,427,527 to 3,428,453 _15620
Group Condition PS417_15595 PS417_15605 PS417_15610 PS417_15615 PS417_15620
carbon source L-Valine (C) N.D. +0.2 -1.4 -0.6 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.1 -0.4 -0.4 -0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 -0.7 -0.2 -0.3
phage P. simiae OR1 MOI 0.1 N.D. -0.5 -0.8 +0.2 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 +0.1 -0.4 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.4 -0.2 +0.1 -0.5
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. +0.1 -0.3 +0.2 -1.0
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.1 -0.9 -0.4 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.4 -0.3 +0.2 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.5 -0.5 -0.0 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.1 -0.8 +0.2 -0.2
agar plate interaction control Taped volatile agar plate with no fungus N.D. +0.2 +0.1 +0.1 -1.2
seeds Growth on radish seeds for 72 hours N.D. -0.2 -0.9 +0.4 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 -0.7 -0.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.6 -0.4 -0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 -0.6 +0.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.6 -0.2 +0.2
carbon source L-Glutamine (C) N.D. +0.3 -0.4 +0.2 -0.3
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. -0.4 +0.3 +0.3 -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.1 +0.6 -0.2 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 +0.6 -0.0 -0.3
carbon source L-Lactate (C) N.D. -0.3 +0.6 +0.1 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.4 +1.0 -0.2 -0.0
carbon source L-Valine (C) N.D. -0.2 +0.6 +0.3 -0.3
soil soil sample 4; outgrowth in LB N.D. -0.3 +0.8 +0.1 -0.2
stress Gentamicin 0.008 mg/ml N.D. +0.1 +0.6 -0.1 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.3 +0.5 +0.4 -0.2
rhizosphere rhizosphere sample 8; outgrowth in LB N.D. +0.4 +0.4 -0.0 +0.4
phage JP1 MOI 0.1 N.D. -0.0 +0.6 +0.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.5 +0.8 -0.2 +0.3
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