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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_15575 and PS417_15580 are separated by 109 nucleotides
PS417_15580 and PS417_15585 overlap by 4 nucleotides
PS417_15585 and PS417_15590 are separated by 7 nucleotides
PS417_15590 and PS417_15595 overlap by 1 nucleotides
PS417_15575: PS417_15575 - histidine kinase, at 3,412,786 to 3,414,498
_15575
PS417_15580: PS417_15580 - DNA polymerase, at 3,414,608 to 3,417,679
_15580
PS417_15585: PS417_15585 - DNA repair nucleotidyltransferase, at 3,417,676 to 3,419,088
_15585
PS417_15590: PS417_15590 - hypothetical protein, at 3,419,096 to 3,419,710
_15590
PS417_15595: PS417_15595 - LexA family transcriptional regulator, at 3,419,710 to 3,420,327
_15595
Group
Condition
PS417
_15575
PS417
_15580
PS417
_15585
PS417
_15590
PS417
_15595
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.2
-0.0
-1.2
-0.5
N.D.
motility
inner cut, LB soft agar motility assay
-0.3
+0.1
-1.1
-0.3
N.D.
phage
P. simiae ORA MOI 0.1
-0.2
-0.4
-0.5
-0.6
N.D.
phage
P. simiae ORA MOI 1
-0.1
-0.2
-0.7
-0.6
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.3
-0.1
-0.6
-0.5
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.1
-1.0
-0.2
N.D.
phage
P. simiae Grant Run MOI 0.1
+0.1
+0.4
-0.8
-0.6
N.D.
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.1
+0.4
-0.5
-0.6
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
+0.1
-0.2
-1.1
+0.4
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.3
+0.6
-0.2
-0.6
N.D.
phage
P. simiae OR Antrim MOI 0.1
+0.0
+0.4
-0.7
-0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
-0.4
-0.4
+0.5
N.D.
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.4
-0.0
+0.5
-0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-0.2
-0.6
+0.1
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
+0.2
-0.7
+0.5
N.D.
phage
JP1 MOI 0.1
+0.4
-0.3
+0.9
-1.0
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.4
+0.1
+0.3
-0.6
N.D.
carbon source
L-Valine (C)
-0.5
-0.3
+0.9
+0.3
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.4
+0.7
-0.2
+0.1
N.D.
phage
JP1 MOI 1
+0.2
+0.0
+1.1
-0.9
N.D.
seeds
Growth on radish seeds for 72 hours
-0.2
+0.4
+0.8
-0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.4
+0.5
+0.9
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+0.2
-0.1
+1.0
N.D.
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.0
+0.1
+1.3
-0.3
N.D.
rhizosphere
rhizosphere sample 1; outgrowth in LB
-0.1
+0.6
+0.7
+0.2
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
-0.0
+0.6
+0.6
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
+0.5
+1.0
+0.0
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
+0.2
-0.2
+1.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+0.1
+0.7
+1.0
N.D.
phage
P. simiae OR1 MOI 0.1
+0.1
-0.1
+1.0
+0.8
N.D.
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