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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_15535 and PS417_15540 are separated by 127 nucleotides
PS417_15540 and PS417_15545 are separated by 44 nucleotides
PS417_15545 and PS417_15550 are separated by 69 nucleotides
PS417_15550 and PS417_15555 are separated by 289 nucleotides
PS417_15535: PS417_15535 - oxidoreductase, at 3,400,289 to 3,401,269
_15535
PS417_15540: PS417_15540 - NUDIX hydrolase, at 3,401,397 to 3,401,759
_15540
PS417_15545: PS417_15545 - glucosidase, at 3,401,804 to 3,404,440
_15545
PS417_15550: PS417_15550 - porin, at 3,404,510 to 3,405,919
_15550
PS417_15555: PS417_15555 - hypothetical protein, at 3,406,209 to 3,407,585
_15555
Group
Condition
PS417
_15535
PS417
_15540
PS417
_15545
PS417
_15550
PS417
_15555
soil
soil sample 4; outgrowth in LB
-0.9
-1.8
+0.1
-0.2
-0.1
motility
outer cut, LB soft agar motility assay
-0.9
-1.5
+0.0
-0.0
-0.0
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.4
-2.7
-0.1
-0.1
+0.4
nophagecontrol
TIme6-nophage
-0.5
-1.6
+0.1
-0.1
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-2.0
+0.1
+0.1
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
-1.9
+0.1
-0.2
+0.6
soil
soil sample 7; outgrowth in LB
+0.1
-1.6
-0.2
-0.2
+0.3
phage
P. simiae OR1 MOI 0.1
+0.4
-1.5
+0.1
+0.3
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.7
-1.7
-0.1
+0.2
-0.5
agar plate interaction control
Taped volatile agar plate with no fungus
-0.1
-1.0
-0.3
+0.2
+0.6
seeds
Growth on radish seeds for 72 hours
-0.3
-0.8
+0.4
-0.3
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.8
-1.2
-0.2
-0.0
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.5
-1.2
+0.0
-0.0
+0.5
phage
P. simiae OR Antrim MOI 1
+0.6
-0.4
+0.4
-0.2
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-1.0
+0.4
+0.1
-0.1
+0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+1.1
-0.6
-0.2
-0.1
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+2.1
-0.3
+0.1
-0.4
carbon source
2'-Deoxyinosine 5 mM (C)
-0.3
+1.5
+0.1
-0.1
-0.3
phage
JP1 MOI 1
-0.3
+1.2
-0.0
+0.3
-0.2
stress
methylglyoxal 0.032 vol%
+1.3
+0.5
-0.1
-0.4
-0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.7
+0.9
+0.1
+0.4
+0.4
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.0
+1.5
-0.1
+0.2
-0.3
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+1.4
-0.4
+0.6
-0.2
+0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.6
+2.1
+0.1
-0.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
+2.1
-0.1
-0.1
-0.1
phage
P. simiae Grant Run MOI 0.1
+0.5
+1.0
-0.1
+0.4
+0.1
soil
soil sample 2; outgrowth in LB
+0.5
+1.1
-0.1
+0.3
+0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.3
+2.0
+0.3
-0.0
-0.2
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+2.7
+0.1
+0.0
+0.1
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.3
+1.7
-0.1
+0.3
+0.3
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