Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15525 and PS417_15530 overlap by 10 nucleotidesPS417_15530 and PS417_15535 are separated by 36 nucleotidesPS417_15535 and PS417_15540 are separated by 127 nucleotidesPS417_15540 and PS417_15545 are separated by 44 nucleotides PS417_15525: PS417_15525 - AraC family transcriptional regulator, at 3,398,766 to 3,399,632 _15525 PS417_15530: PS417_15530 - ribonuclease, at 3,399,623 to 3,400,252 _15530 PS417_15535: PS417_15535 - oxidoreductase, at 3,400,289 to 3,401,269 _15535 PS417_15540: PS417_15540 - NUDIX hydrolase, at 3,401,397 to 3,401,759 _15540 PS417_15545: PS417_15545 - glucosidase, at 3,401,804 to 3,404,440 _15545
Group Condition PS417_15525 PS417_15530 PS417_15535 PS417_15540 PS417_15545
soil soil sample 4; outgrowth in LB -0.7 +0.0 -0.9 -1.8 +0.1
motility outer cut, LB soft agar motility assay +0.0 -0.1 -0.9 -1.5 +0.0
rhizosphere rhizosphere sample 2; outgrowth in LB +0.0 +0.2 +0.4 -2.7 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.2 +0.3 -2.0 +0.1
nophagecontrol TIme6-nophage +0.0 -0.1 -0.5 -1.6 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 +0.3 -0.3 -1.5 -0.1
stress R2A with Fusaric 120 ug/mL -0.8 +0.1 -0.4 -0.8 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 +0.3 -0.3 -1.9 +0.1
stress 1-ethyl-3-methylimidazolium chloride 200 mM -0.5 +0.1 +0.2 -1.4 -0.1
agar plate interaction control Taped volatile agar plate with no fungus +0.2 -0.2 +0.2 -1.4 -0.3
phage P. simiae OR1 MOI 0.1 -0.1 -0.3 +0.4 -1.5 +0.1
seeds Growth on radish seeds for 72 hours -0.7 +0.2 -0.3 -0.8 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.3 +0.7 -1.7 -0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 -0.3 -0.1 -1.2 +0.1
soil soil sample 7; outgrowth in LB +0.6 +0.3 +0.1 -1.6 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 -0.3 +0.8 -1.2 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 -0.1 +1.1 -0.6 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.5 -0.6 +1.1 -0.3
carbon source 2-Deoxy-D-Ribose 10 mM (C) +0.4 +0.3 -1.0 +0.4 -0.1
soil soil sample 2; outgrowth in LB -0.5 -0.2 +0.5 +1.1 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.1 -0.7 +2.1 -0.3
phage P. simiae Grant Run MOI 0.1 +0.2 -0.3 +0.5 +1.0 -0.1
carbon source 2'-Deoxyinosine 5 mM (C) +0.4 -0.1 -0.3 +1.5 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.8 +0.5 +0.2 -0.4 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 +0.2 -0.6 +2.1 +0.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.1 +1.4 -0.4 +0.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 +0.2 +0.3 +2.0 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 +0.0 +1.3 +1.7 -0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 +0.0 +2.7 +0.1 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 +0.4 -0.1 +2.1 -0.1
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