Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15425 and PS417_15430 are separated by 170 nucleotidesPS417_15430 and PS417_15435 are separated by 7 nucleotidesPS417_15435 and PS417_15440 are separated by 9 nucleotidesPS417_15440 and PS417_15445 overlap by 4 nucleotides PS417_15425: PS417_15425 - acetyltransferase, at 3,380,711 to 3,381,169 _15425 PS417_15430: PS417_15430 - porin, at 3,381,340 to 3,382,659 _15430 PS417_15435: PS417_15435 - metallophosphatase, at 3,382,667 to 3,383,500 _15435 PS417_15440: PS417_15440 - iron ABC transporter substrate-binding protein, at 3,383,510 to 3,384,541 _15440 PS417_15445: PS417_15445 - ABC transporter permease, at 3,384,538 to 3,385,332 _15445
Group Condition PS417_15425 PS417_15430 PS417_15435 PS417_15440 PS417_15445
phage P. simiae Grant Run MOI 10 -0.0 +0.2 -0.7 -0.8 -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.0 +0.1 -2.0 +0.9 -0.1
phage P. simiae ORA MOI 1 +0.5 -0.1 -0.6 -0.6 +0.1
phage P. simiae OR Antrim MOI 10 -1.1 +0.0 -0.3 +0.5 +0.2
no phage control Time6-nophage -0.5 -0.3 -0.5 +0.3 +0.4
soil soil sample 3; outgrowth in LB -0.6 -0.3 +0.8 +0.2 -0.6
agar plate interaction control Taped volatile agar plate with no fungus -0.7 -0.5 +0.4 +0.7 -0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 -0.1 -0.9 +0.9 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.1 -0.8 +0.6 +0.2
phage P. simiae Grant Run MOI 10 -0.8 +0.1 +0.5 +0.3 -0.4
phage Wcs_0.1 +0.4 +0.0 -1.0 +0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.1 -0.9 -0.1 +0.5
seeds Growth on radish seeds for 24 hours +0.9 -0.0 +0.2 -0.8 +0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.5 -0.3 +0.1 -0.5 +0.6
stress Nalidixic 0.015 mg/ml +0.6 +0.2 -0.7 +0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 +0.2 -0.4 -0.3 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB +0.4 +0.0 -0.6 +0.6 +0.4
supernatant control Vogels_fungal_media 0.1X +0.2 -0.1 +0.5 -0.4 +0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.3 -0.0 +0.2 +1.3 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.6 -0.3 -0.2 +0.3 +0.6
seeds Growth on radish seeds for 72 hours +1.1 -0.4 +0.1 +0.1 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.1 +0.2 -0.5 +0.8
carbon source L-Valine (C) +0.4 +0.1 +1.1 +0.0 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 +0.2 +0.7 -0.4 +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.8 -0.2 +0.0 +0.4 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.6 -0.1 +0.7 +0.1 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.9 -0.1 +0.1 +0.6 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.2 -0.2 +0.2 +1.8 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.9 -0.3 +1.0 +0.9 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 +0.1 +0.3 +1.1 +1.8
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