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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_15395 and PS417_15400 are separated by 131 nucleotides
PS417_15400 and PS417_15405 overlap by 26 nucleotides
PS417_15405 and PS417_15410 are separated by 96 nucleotides
PS417_15410 and PS417_15415 are separated by 292 nucleotides
PS417_15395: PS417_15395 - ATPase, at 3,376,069 to 3,376,713
_15395
PS417_15400: PS417_15400 - hypothetical protein, at 3,376,845 to 3,377,117
_15400
PS417_15405: PS417_15405 - glutathione S-transferase, at 3,377,092 to 3,377,709
_15405
PS417_15410: PS417_15410 - hypothetical protein, at 3,377,806 to 3,378,354
_15410
PS417_15415: PS417_15415 - membrane protein, at 3,378,647 to 3,379,801
_15415
Group
Condition
PS417
_15395
PS417
_15400
PS417
_15405
PS417
_15410
PS417
_15415
soil
soil sample 5; outgrowth in LB
-0.2
-1.8
-0.3
-0.6
-0.3
no phage control
Time6-nophage
-0.0
-1.8
-0.3
-0.2
-0.3
seeds
Growth on radish seeds for 72 hours
-0.5
-1.1
-0.2
-0.1
-0.3
soil
soil sample 2; outgrowth in LB
-0.0
-1.4
-0.2
+0.3
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
-0.6
-0.7
+0.2
-0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.3
-0.7
-0.1
-1.3
+0.3
phage
JP1 MOI 10
-0.0
-1.3
-0.3
-0.6
+0.8
stress
Polymyxin B sulfate 0.006 mg/ml
-0.0
-1.3
-0.3
+0.3
-0.2
phage
JP1 MOI 1
+0.1
-1.1
+0.0
-0.5
+0.4
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
+0.2
-1.5
+0.2
-0.3
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+0.1
-0.7
-0.6
+0.4
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
+0.1
-1.2
+0.3
-0.4
+0.3
phage
JP1 MOI 0.1
-0.2
-1.2
-0.0
-0.1
+0.6
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
+0.1
-0.9
+0.3
-0.6
+0.3
motility
outer cut, LB soft agar motility assay
+0.2
-1.2
-0.2
+0.4
+0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
-1.0
+0.0
-0.3
+0.7
supernatant control
Vogels_fungal_media 0.1X
+0.1
-0.9
-0.3
+0.7
+0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
-0.9
-0.3
+0.9
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+1.2
-0.2
-0.4
-0.0
ecofab
LB 0.5x in EcoFAB_3.5mL
-0.2
+0.6
-0.1
+0.7
-0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.3
+0.4
-0.4
+0.6
-0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-0.2
+0.5
+0.1
+1.0
-0.2
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
-0.1
+1.4
+0.3
+0.4
-0.6
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+1.5
-0.5
+0.1
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.1
+1.0
-0.1
-0.3
+0.9
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+1.5
-0.2
+0.0
+0.0
stress
Gentamicin 0.008 mg/ml
+0.2
+1.4
+0.5
+0.1
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.6
+0.5
+0.5
+0.4
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.2
+0.9
-0.1
+0.1
+0.9
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.0
-0.0
-0.3
+1.9
+0.3
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