Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15395 and PS417_15400 are separated by 131 nucleotidesPS417_15400 and PS417_15405 overlap by 26 nucleotidesPS417_15405 and PS417_15410 are separated by 96 nucleotidesPS417_15410 and PS417_15415 are separated by 292 nucleotides PS417_15395: PS417_15395 - ATPase, at 3,376,069 to 3,376,713 _15395 PS417_15400: PS417_15400 - hypothetical protein, at 3,376,845 to 3,377,117 _15400 PS417_15405: PS417_15405 - glutathione S-transferase, at 3,377,092 to 3,377,709 _15405 PS417_15410: PS417_15410 - hypothetical protein, at 3,377,806 to 3,378,354 _15410 PS417_15415: PS417_15415 - membrane protein, at 3,378,647 to 3,379,801 _15415
Group Condition PS417_15395 PS417_15400 PS417_15405 PS417_15410 PS417_15415
soil soil sample 5; outgrowth in LB -0.2 -1.8 -0.3 -0.6 -0.3
no phage control Time6-nophage -0.0 -1.8 -0.3 -0.2 -0.3
seeds Growth on radish seeds for 72 hours -0.5 -1.1 -0.2 -0.1 -0.3
soil soil sample 2; outgrowth in LB -0.0 -1.4 -0.2 +0.3 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 -0.6 -0.7 +0.2 -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.3 -0.7 -0.1 -1.3 +0.3
phage JP1 MOI 10 -0.0 -1.3 -0.3 -0.6 +0.8
stress Polymyxin B sulfate 0.006 mg/ml -0.0 -1.3 -0.3 +0.3 -0.2
phage JP1 MOI 1 +0.1 -1.1 +0.0 -0.5 +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.2 -1.5 +0.2 -0.3 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.1 -0.7 -0.6 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.1 -1.2 +0.3 -0.4 +0.3
phage JP1 MOI 0.1 -0.2 -1.2 -0.0 -0.1 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.1 -0.9 +0.3 -0.6 +0.3
motility outer cut, LB soft agar motility assay +0.2 -1.2 -0.2 +0.4 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -1.0 +0.0 -0.3 +0.7
supernatant control Vogels_fungal_media 0.1X +0.1 -0.9 -0.3 +0.7 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.9 -0.3 +0.9 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +1.2 -0.2 -0.4 -0.0
ecofab LB 0.5x in EcoFAB_3.5mL -0.2 +0.6 -0.1 +0.7 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +0.4 -0.4 +0.6 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.2 +0.5 +0.1 +1.0 -0.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.1 +1.4 +0.3 +0.4 -0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +1.5 -0.5 +0.1 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 +1.0 -0.1 -0.3 +0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +1.5 -0.2 +0.0 +0.0
stress Gentamicin 0.008 mg/ml +0.2 +1.4 +0.5 +0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.6 +0.5 +0.5 +0.4 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 +0.9 -0.1 +0.1 +0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 -0.0 -0.3 +1.9 +0.3
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