Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15320 and PS417_15325 are separated by 28 nucleotidesPS417_15325 and PS417_15330 are separated by 31 nucleotidesPS417_15330 and PS417_15335 are separated by 478 nucleotidesPS417_15335 and PS417_15340 are separated by 52 nucleotides PS417_15320: PS417_15320 - type VI secretion protein, at 3,361,717 to 3,363,192 _15320 PS417_15325: PS417_15325 - type VI secretion protein, at 3,363,221 to 3,363,718 _15325 PS417_15330: PS417_15330 - type VI secretion protein ImpA, at 3,363,750 to 3,365,303 _15330 PS417_15335: PS417_15335 - hypothetical protein, at 3,365,782 to 3,366,297 _15335 PS417_15340: PS417_15340 - oxidoreductase, at 3,366,350 to 3,367,171 _15340
Group Condition PS417_15320 PS417_15325 PS417_15330 PS417_15335 PS417_15340
seeds Growth on radish seeds for 24 hours +0.2 -2.5 -0.4 -1.0 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 -3.0 -0.3 +0.1 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.1 -0.4 -1.4 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.6 -0.3 -1.1 -0.2
seeds Growth on radish seeds for 72 hours +0.6 -1.5 -0.3 -0.4 -0.2
stress Gentamicin 0.008 mg/ml +0.1 -1.6 -0.0 +0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -1.2 -0.6 +0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 -1.1 -0.3 +0.6 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +0.2 -1.0 +0.5 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -1.6 -0.3 +0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -1.1 -0.5 +0.9 -0.3
soil soil sample 5; outgrowth in LB -0.1 -1.3 +0.2 +1.5 -0.5
soil soil sample 7; outgrowth in LB +0.1 -2.0 -0.1 +1.3 +0.5
phage JP1 MOI 10 +0.2 -1.2 +0.1 +0.8 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +1.0 -0.1 +0.1 -0.6
phage P. simiae Grant Run MOI 0.1 -0.1 +0.8 -0.6 +0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.2 +1.4 -0.3 -0.4 +0.0
soil soil sample 3; outgrowth in LB +0.1 -1.1 -0.0 +1.0 +0.5
rhizosphere rhizosphere sample 2; outgrowth in LB +0.3 -1.3 -0.2 +2.2 -0.4
phage P. simiae OR1 MOI 0.1 -0.1 +1.3 -1.4 +0.3 +0.6
soil soil sample 8; outgrowth in LB +0.1 -0.9 +0.2 +1.2 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 -0.7 +0.4 +0.2 +0.4
rhizosphere rhizosphere sample 1; outgrowth in LB -0.1 +0.1 -0.4 +1.9 -0.3
soil soil sample 4; outgrowth in LB -0.1 -0.0 -0.6 +1.3 +0.7
root root sample 6; outgrowth in LB -0.5 -0.3 -0.3 +2.4 +0.2
soil soil sample 2; outgrowth in LB -0.1 -0.3 +0.3 +1.3 +0.4
phage P. simiae ORA MOI 0.1 +0.3 +1.1 +0.6 -0.4 +0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 +1.5 -0.2 -0.0 +0.6
rhizosphere rhizosphere sample 8; outgrowth in LB +0.2 +0.9 -0.0 +2.0 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 +2.2 -0.1 +1.5 +0.4
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