Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15190 and PS417_15200 are separated by 1279 nucleotidesPS417_15200 and PS417_15205 are separated by 412 nucleotidesPS417_15205 and PS417_15210 are separated by 130 nucleotidesPS417_15210 and PS417_15215 are separated by 132 nucleotides PS417_15190: PS417_15190 - acyl-CoA dehydrogenase, at 3,329,224 to 3,331,749 _15190 PS417_15200: PS417_15200 - protein activator of alkane oxidation PraB, at 3,333,029 to 3,333,523 _15200 PS417_15205: PS417_15205 - membrane protein, at 3,333,936 to 3,335,390 _15205 PS417_15210: PS417_15210 - protein activator of alkane oxidation PraB, at 3,335,521 to 3,336,015 _15210 PS417_15215: PS417_15215 - protein activator of alkane oxidation PraA, at 3,336,148 to 3,336,642 _15215
Group Condition PS417_15190 PS417_15200 PS417_15205 PS417_15210 PS417_15215
rhizosphere rhizosphere sample 2; outgrowth in LB -0.0 -2.2 +0.1 -0.2 -1.4
soil soil sample 6; outgrowth in LB -0.3 +0.0 -0.1 +0.2 -2.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.2 -0.6 -0.1 -1.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -1.6 -0.4 +0.6 -0.7
motility outer cut, LB soft agar motility assay -0.2 -1.1 +0.2 -0.2 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.0 -0.3 +0.2 +0.2 -1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.3 -0.2 +0.6 -2.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.0 -0.3 +0.6 -1.6
phage P. simiae OR1 MOI 0.1 +0.1 +1.1 -0.1 +0.1 -2.2
soil soil sample 8; outgrowth in LB -0.0 -0.4 -0.1 -1.1 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.0 -0.5 +0.4 +0.4 -1.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.8 -0.1 -0.2 -1.5
phage P. simiae OR Antrim MOI 1 +0.1 +0.3 +0.3 -0.3 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -1.2 -0.3 +0.1 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.8 -0.1 -0.5 +0.8
agar plate interaction control Taped volatile agar plate with no fungus -0.2 +1.0 -0.0 -0.3 -0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -1.4 +0.2 +0.7 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 -0.9 -0.0 +1.0 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 -0.3 -0.2 -0.4 +1.2
phage JP1 MOI 0.1 +0.1 -1.0 +0.4 -0.1 +0.9
supernatant control Vogels_fungal_media 0.1X +0.0 -0.6 +0.2 +0.1 +1.3
phage P. simiae OR Antrim MOI 1 +0.1 +1.6 +0.3 -0.1 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 +0.9 -0.3 +0.3 +0.7
soil soil sample 5; outgrowth in LB +0.5 +1.1 -0.2 +0.3 -0.0
phage P. simiae Grant Run MOI 0.1 +0.2 +0.5 +0.1 +0.0 +1.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 +0.3 +0.1 +0.5 +1.1
phage P. simiae ORA MOI 0.1 +0.2 +1.2 +0.3 +0.5 +0.0
carbon source L-Valine (C) -0.1 +1.0 +0.3 +0.8 +0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.0 +0.9 -0.1 +1.3 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 +0.9 -0.0 -0.5 +2.4
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