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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_15185 and PS417_15190 are separated by 148 nucleotides
PS417_15190 and PS417_15200 are separated by 1279 nucleotides
PS417_15200 and PS417_15205 are separated by 412 nucleotides
PS417_15205 and PS417_15210 are separated by 130 nucleotides
PS417_15185: PS417_15185 - 3-ketoacyl-ACP reductase, at 3,327,732 to 3,329,075
_15185
PS417_15190: PS417_15190 - acyl-CoA dehydrogenase, at 3,329,224 to 3,331,749
_15190
PS417_15200: PS417_15200 - protein activator of alkane oxidation PraB, at 3,333,029 to 3,333,523
_15200
PS417_15205: PS417_15205 - membrane protein, at 3,333,936 to 3,335,390
_15205
PS417_15210: PS417_15210 - protein activator of alkane oxidation PraB, at 3,335,521 to 3,336,015
_15210
Group
Condition
PS417
_15185
PS417
_15190
PS417
_15200
PS417
_15205
PS417
_15210
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.0
-0.0
-2.2
+0.1
-0.2
soil
soil sample 8; outgrowth in LB
-0.1
-0.0
-0.4
-0.1
-1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.1
-1.2
-0.3
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.1
-1.6
-0.4
+0.6
motility
outer cut, LB soft agar motility assay
-0.2
-0.2
-1.1
+0.2
-0.2
seeds
Growth on radish seeds for 72 hours
+0.3
-0.1
-1.0
+0.3
-0.5
root
root sample 6; outgrowth in LB
+0.4
-0.1
-0.2
+0.2
-1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
+0.2
-0.8
-0.1
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
+0.0
-1.4
+0.2
+0.7
phage
JP1 MOI 0.1
+0.1
+0.1
-1.0
+0.4
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.2
-0.0
-0.5
+0.4
+0.4
no phage control
Time6-nophage
-0.2
-0.0
+0.6
+0.3
-0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.4
-0.3
-0.9
-0.0
+1.0
phage
FRSphage
-0.6
-0.1
+0.9
-0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
+0.2
+0.8
-0.1
-0.2
agar plate interaction control
Taped volatile agar plate with no fungus
-0.1
-0.2
+1.0
-0.0
-0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.3
-0.2
+0.7
-0.2
+0.5
carbon source
octanoate (C)
+0.3
+0.1
-0.5
+0.2
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
-0.1
+0.9
-0.3
+0.3
seeds
Growth on radish seeds for 72 hours
-0.1
+0.2
+0.8
+0.2
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
+0.1
-0.0
-0.3
+0.6
stress
LB_noSalt with Chloride 500 mM
-0.1
+0.1
+1.1
+0.2
-0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.1
+0.2
+1.2
-0.0
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+0.3
-0.0
+0.3
+1.0
soil
soil sample 5; outgrowth in LB
-0.1
+0.5
+1.1
-0.2
+0.3
phage
P. simiae OR1 MOI 0.1
+0.4
+0.1
+1.1
-0.1
+0.1
carbon source
L-Valine (C)
-0.2
-0.1
+1.0
+0.3
+0.8
phage
P. simiae OR Antrim MOI 1
-0.1
+0.1
+1.6
+0.3
-0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
-0.0
+0.9
-0.1
+1.3
phage
P. simiae ORA MOI 0.1
-0.0
+0.2
+1.2
+0.3
+0.5
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