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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_15175 and PS417_15180 overlap by 4 nucleotides
PS417_15180 and PS417_15185 are separated by 3 nucleotides
PS417_15185 and PS417_15190 are separated by 148 nucleotides
PS417_15190 and PS417_15200 are separated by 1279 nucleotides
PS417_15175: PS417_15175 - acetyl-CoA acetyltransferase, at 3,325,543 to 3,326,841
_15175
PS417_15180: PS417_15180 - acyl dehydratase, at 3,326,838 to 3,327,728
_15180
PS417_15185: PS417_15185 - 3-ketoacyl-ACP reductase, at 3,327,732 to 3,329,075
_15185
PS417_15190: PS417_15190 - acyl-CoA dehydrogenase, at 3,329,224 to 3,331,749
_15190
PS417_15200: PS417_15200 - protein activator of alkane oxidation PraB, at 3,333,029 to 3,333,523
_15200
Group
Condition
PS417
_15175
PS417
_15180
PS417
_15185
PS417
_15190
PS417
_15200
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.7
-0.1
-0.1
-1.6
rhizosphere
rhizosphere sample 2; outgrowth in LB
N.D.
+0.2
+0.0
-0.0
-2.2
motility
outer cut, LB soft agar motility assay
N.D.
-0.2
-0.2
-0.2
-1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Phenylalanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-0.6
-0.1
-0.1
-0.7
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
-0.5
-0.2
-0.1
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.0
-0.1
-0.1
-1.2
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
N.D.
-0.3
-0.1
-0.2
-0.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.5
-0.3
+0.0
-1.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
N.D.
-0.3
+0.4
-0.3
-0.9
stress
Nalidixic 0.025 mg/ml
N.D.
+0.3
+0.0
-0.1
-1.0
rhizosphere
rhizosphere sample 8; outgrowth in LB
N.D.
+0.3
+0.1
+0.1
-1.1
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
-0.7
+0.1
-0.3
+0.3
seeds
Growth on radish seeds for 72 hours
N.D.
+0.2
+0.3
-0.1
-1.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
+0.7
-0.2
-0.2
-0.6
phage
JP1 MOI 0.1
N.D.
+0.5
+0.1
+0.1
-1.0
phage
Wcs_1
N.D.
-0.7
+0.0
-0.1
+0.5
phage
FRSphage
N.D.
-0.3
-0.6
-0.1
+0.9
phage
P. simiae Grant Run MOI 10
N.D.
-0.6
+0.1
+0.1
+0.6
soil
soil sample 3; outgrowth in LB
N.D.
-0.6
+0.2
-0.0
+0.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
-0.5
-0.0
+0.0
+0.9
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
-0.3
-0.1
-0.2
+1.0
carbon source
L-Valine (C)
N.D.
-0.1
-0.2
-0.1
+1.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
-0.2
-0.1
+0.2
+1.2
phage
P. simiae OR1 MOI 0.1
N.D.
-0.4
+0.4
+0.1
+1.1
seeds
Growth on radish seeds for 72 hours
N.D.
+0.4
-0.1
+0.2
+0.8
soil
soil sample 5; outgrowth in LB
N.D.
-0.2
-0.1
+0.5
+1.1
stress
LB_noSalt with Chloride 500 mM
N.D.
+0.3
-0.1
+0.1
+1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.6
-0.3
+0.2
+0.8
phage
P. simiae ORA MOI 0.1
N.D.
+0.1
-0.0
+0.2
+1.2
phage
P. simiae OR Antrim MOI 1
N.D.
-0.0
-0.1
+0.1
+1.6
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