Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15145 and PS417_15150 are separated by 136 nucleotidesPS417_15150 and PS417_15155 are separated by 39 nucleotidesPS417_15155 and PS417_15160 are separated by 15 nucleotidesPS417_15160 and PS417_15165 are separated by 306 nucleotides PS417_15145: PS417_15145 - hypothetical protein, at 3,313,478 to 3,313,768 _15145 PS417_15150: PS417_15150 - hypothetical protein, at 3,313,905 to 3,318,815 _15150 PS417_15155: PS417_15155 - RND transporter, at 3,318,855 to 3,321,230 _15155 PS417_15160: PS417_15160 - hypothetical protein, at 3,321,246 to 3,322,274 _15160 PS417_15165: PS417_15165 - 1-carboxy-3-chloro-3,4-dihydroxycyclo hexa-1,5-diene dehydrogenase, at 3,322,581 to 3,323,696 _15165
Group Condition PS417_15145 PS417_15150 PS417_15155 PS417_15160 PS417_15165
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 +0.2 -1.1 -0.4
soil soil sample 2; outgrowth in LB N.D. -0.1 +0.1 -1.4 -0.0
seeds Growth on radish seeds for 72 hours N.D. -0.3 -0.0 -0.5 -0.5
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. +0.1 -0.5 -0.9 -0.1
solid stress 80% Methanol 3 mM; solid stress N.D. +0.1 -0.0 -1.2 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 -0.1 -0.9 -0.4
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.2 +0.1 -0.4 -0.7
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. +0.1 -0.0 -0.8 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 -0.0 -1.0 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 +0.3 -0.9 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. +0.1 +0.3 -0.7 -0.3
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.1 +0.5 -0.4 -0.4
stress Doxycycline hyclate 0.001 mg/ml N.D. +0.2 +0.2 -0.7 +0.2
phage JP1 MOI 1 N.D. +0.3 +0.1 -0.6 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.1 +0.1 +0.7 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 -0.1 +0.7 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.0 -0.2 +1.2 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.1 -0.3 +1.0 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.0 -0.1 +0.9 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.3 +0.2 +0.9 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -0.1 +1.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.2 +0.1 +0.9 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 +0.2 +1.1 -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.3 -0.1 +0.6 +0.3
soil soil sample 7; outgrowth in LB N.D. +0.4 +0.2 +0.7 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 +0.3 +1.1 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 +0.2 +1.5 -0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.2 +0.2 +0.9 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.2 +0.3 +0.9 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.3 +0.1 +2.1 -0.4
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