Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15140 and PS417_15145 overlap by 14 nucleotidesPS417_15145 and PS417_15150 are separated by 136 nucleotidesPS417_15150 and PS417_15155 are separated by 39 nucleotidesPS417_15155 and PS417_15160 are separated by 15 nucleotides PS417_15140: PS417_15140 - 3-carboxymuconate cyclase, at 3,312,316 to 3,313,491 _15140 PS417_15145: PS417_15145 - hypothetical protein, at 3,313,478 to 3,313,768 _15145 PS417_15150: PS417_15150 - hypothetical protein, at 3,313,905 to 3,318,815 _15150 PS417_15155: PS417_15155 - RND transporter, at 3,318,855 to 3,321,230 _15155 PS417_15160: PS417_15160 - hypothetical protein, at 3,321,246 to 3,322,274 _15160
Group Condition PS417_15140 PS417_15145 PS417_15150 PS417_15155 PS417_15160
soil soil sample 2; outgrowth in LB -0.6 N.D. -0.1 +0.1 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 N.D. -0.1 +0.2 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 N.D. +0.1 -0.1 -0.9
soil soil sample 6; outgrowth in LB -0.9 N.D. -0.1 +0.1 -0.7
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -0.6 N.D. +0.1 -0.0 -0.8
solid stress 80% Methanol 3 mM; solid stress -0.1 N.D. +0.1 -0.0 -1.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -0.0 N.D. +0.1 -0.5 -0.9
soil soil sample 3; outgrowth in LB -1.0 N.D. +0.2 -0.1 +0.1
soil soil sample 4; outgrowth in LB -0.5 N.D. +0.1 +0.3 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 N.D. +0.3 -0.0 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 N.D. -0.4 -0.1 +0.7
seeds Growth on radish seeds for 72 hours +0.6 N.D. -0.3 -0.0 -0.5
root root sample 6; outgrowth in LB -0.9 N.D. +0.1 +0.2 +0.3
phage P. simiae ORA MOI 0.1 -0.6 N.D. +0.0 -0.2 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. -0.1 -0.3 +1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.4 N.D. -0.1 -0.0 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. +0.0 -0.2 +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 N.D. -0.3 +0.2 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.5 N.D. -0.2 +0.1 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.5 N.D. +0.1 +0.1 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 N.D. -0.3 -0.1 +1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 N.D. +0.0 -0.2 +1.2
soil soil sample 7; outgrowth in LB -0.4 N.D. +0.4 +0.2 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 N.D. -0.2 +0.2 +1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -0.2 +0.3 +1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.3 N.D. -0.0 +0.1 +0.9
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 N.D. +0.2 +0.2 +0.9
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.3 N.D. +0.1 +0.1 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 N.D. +0.1 +0.2 +1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 N.D. +0.3 +0.1 +2.1
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