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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_15045 and PS417_15050 are separated by 3 nucleotides
PS417_15050 and PS417_15055 are separated by 232 nucleotides
PS417_15055 and PS417_15060 are separated by 172 nucleotides
PS417_15060 and PS417_15065 are separated by 17 nucleotides
PS417_15045: PS417_15045 - cupin, at 3,288,482 to 3,288,883
_15045
PS417_15050: PS417_15050 - 3-methyladenine DNA glycosylase, at 3,288,887 to 3,289,516
_15050
PS417_15055: PS417_15055 - C4-dicarboxylate ABC transporter, at 3,289,749 to 3,291,059
_15055
PS417_15060: PS417_15060 - GntR family transcriptional regulator, at 3,291,232 to 3,291,933
_15060
PS417_15065: PS417_15065 - GntR family transcriptional regulator, at 3,291,951 to 3,292,643
_15065
Group
Condition
PS417
_15045
PS417
_15050
PS417
_15055
PS417
_15060
PS417
_15065
soil
soil sample 4; outgrowth in LB
-1.2
+0.0
-0.2
N.D.
-0.5
phage
P. simiae OR Antrim MOI 10
-0.5
-0.7
-0.3
N.D.
-0.2
carbon source
2'-Deoxyinosine 5 mM (C)
-1.0
-0.1
-0.3
N.D.
+0.0
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.5
-0.4
+0.2
N.D.
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
-0.4
-0.3
N.D.
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.0
-0.4
+0.2
N.D.
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.4
+0.2
N.D.
-0.6
seeds
Growth on radish seeds for 24 hours
-1.0
+0.3
-0.1
N.D.
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.4
+0.1
-0.3
N.D.
-0.9
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
-0.2
-0.5
N.D.
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
-0.2
-0.6
N.D.
+0.6
seeds
Growth on radish seeds for 72 hours
+0.4
-0.2
+0.3
N.D.
-0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.3
+0.3
+0.1
N.D.
-0.8
agar plate interaction control
Taped volatile agar plate with no fungus
+0.5
+0.2
-0.5
N.D.
-0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.6
-0.3
+0.5
N.D.
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
-0.7
-0.1
N.D.
+0.2
stress
Gentamicin 0.008 mg/ml
+0.4
+0.4
+0.5
N.D.
-1.2
phage
JP1 MOI 10
-0.6
+0.4
-0.1
N.D.
+0.3
phage
Wcs_0.1
-0.2
-0.4
+0.2
N.D.
+0.6
supernatant control
Vogels_fungal_media 0.1X
-0.7
-0.0
+0.5
N.D.
+0.5
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.4
+0.1
-0.2
N.D.
+0.8
phage
P. simiae OR1 MOI 0.1
-0.2
-0.4
+0.3
N.D.
+0.7
phage
P. simiae ORA MOI 10
-0.1
-0.1
-0.2
N.D.
+1.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.4
-0.1
+1.4
N.D.
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+0.3
-0.3
N.D.
+1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.5
-0.2
+0.3
N.D.
+0.8
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.8
+0.4
-0.1
N.D.
+0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-0.0
+0.2
+0.3
N.D.
+1.2
root
root sample 6; outgrowth in LB
+0.9
+0.0
+0.2
N.D.
+1.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.4
+0.8
-0.2
N.D.
+1.2
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