Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_15010 and PS417_15015 are separated by 184 nucleotidesPS417_15015 and PS417_15020 are separated by 53 nucleotidesPS417_15020 and PS417_15025 are separated by 129 nucleotidesPS417_15025 and PS417_15030 are separated by 9 nucleotides PS417_15010: PS417_15010 - TetR family transcriptional regulator, at 3,282,082 to 3,282,675 _15010 PS417_15015: PS417_15015 - isochorismatase, at 3,282,860 to 3,283,384 _15015 PS417_15020: PS417_15020 - 2-hydroxyacid dehydrogenase, at 3,283,438 to 3,284,391 _15020 PS417_15025: PS417_15025 - aminoglycoside phosphotransferase, at 3,284,521 to 3,285,588 _15025 PS417_15030: PS417_15030 - short-chain dehydrogenase, at 3,285,598 to 3,286,365 _15030
Group Condition PS417_15010 PS417_15015 PS417_15020 PS417_15025 PS417_15030
soil soil sample 2; outgrowth in LB -0.4 N.D. -0.6 +0.3 -2.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.4 N.D. -0.3 -0.4 -1.2
rhizosphere rhizosphere sample 1; outgrowth in LB -0.3 N.D. -0.6 -0.5 -0.7
rhizosphere rhizosphere sample 2; outgrowth in LB -0.2 N.D. -0.1 +0.1 -1.8
agar plate interaction control Taped volatile agar plate with no fungus +0.1 N.D. -0.3 -0.1 -1.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 N.D. -0.3 -0.2 -1.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.0 N.D. -0.3 -0.1 -1.1
phage P. simiae ORA MOI 0.1 -0.1 N.D. -0.5 +0.3 -1.0
stress Gentamicin 0.008 mg/ml -0.3 N.D. -0.1 +0.3 -1.2
phage P. simiae OR Antrim MOI 0.1 -0.4 N.D. +0.2 +0.1 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 N.D. +0.4 +0.0 -1.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 N.D. -0.1 +0.3 -0.8
phage P. simiae Grant Run MOI 10 -0.3 N.D. +0.5 +0.0 -1.2
stress Nalidixic 0.025 mg/ml -0.1 N.D. +0.2 +0.1 -1.2
phage P. simiae OR1 MOI 0.1 -0.4 N.D. -0.2 +0.6 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 N.D. -0.5 -0.5 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. -0.1 +0.5 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 N.D. -0.3 +0.0 +0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 N.D. +0.5 -0.2 -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 N.D. +0.3 +0.5 -0.8
phage JP1 MOI 1 +0.1 N.D. -0.5 -0.3 +0.8
phage Wcs_1 -0.5 N.D. -0.1 +0.1 +0.9
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 N.D. -0.6 +0.7 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 N.D. -0.3 +0.2 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. +0.3 +0.0 +1.1
motility outer cut, LB soft agar motility assay -0.4 N.D. +0.4 +0.5 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. +0.2 +0.3 +1.1
motility inner cut, LB soft agar motility assay -0.1 N.D. +0.6 +0.1 +0.8
stress Cisplatin 0.01 mg/ml -0.2 N.D. +0.3 +0.3 +1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 N.D. +0.2 +0.4 +1.6
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