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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_14990 and PS417_14995 are separated by 210 nucleotides
PS417_14995 and PS417_15005 are separated by 994 nucleotides
PS417_15005 and PS417_15010 are separated by 151 nucleotides
PS417_15010 and PS417_15015 are separated by 184 nucleotides
PS417_14990: PS417_14990 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 3,276,395 to 3,279,004
_14990
PS417_14995: PS417_14995 - serine/threonine protein kinase, at 3,279,215 to 3,280,492
_14995
PS417_15005: PS417_15005 - hypothetical protein, at 3,281,487 to 3,281,930
_15005
PS417_15010: PS417_15010 - TetR family transcriptional regulator, at 3,282,082 to 3,282,675
_15010
PS417_15015: PS417_15015 - isochorismatase, at 3,282,860 to 3,283,384
_15015
Group
Condition
PS417
_14990
PS417
_14995
PS417
_15005
PS417
_15010
PS417
_15015
stress
Gentamicin 0.008 mg/ml
N.D.
-1.8
-0.3
-0.3
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.9
-1.0
+0.2
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-0.1
-1.3
-0.2
N.D.
soil
soil sample 8; outgrowth in LB
N.D.
-0.2
-1.3
+0.0
N.D.
phage
P. simiae ORA MOI 0.1
N.D.
-0.2
-0.9
-0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.7
-0.2
-0.4
N.D.
phage
JP1 MOI 10
N.D.
-0.3
-1.0
+0.1
N.D.
stress
Cobalt chloride 0.16 mM
N.D.
-1.5
+0.4
-0.1
N.D.
soil
soil sample 4; outgrowth in LB
N.D.
-0.6
-0.6
+0.3
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-0.7
-0.3
+0.3
N.D.
stress
Doxycycline hyclate 0.001 mg/ml
N.D.
+0.3
-0.9
-0.1
N.D.
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
+0.7
-0.8
-0.4
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-0.7
+0.5
-0.1
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.5
-0.8
+0.0
N.D.
liquid stress
Scopoletin 3 mM; liquid stress
N.D.
-0.7
+0.5
+0.0
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.4
-0.7
+0.3
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.5
-0.7
+0.2
N.D.
rhizosphere
rhizosphere sample 8; outgrowth in LB
N.D.
+0.7
-0.6
-0.0
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.1
+0.9
-0.3
N.D.
phage
P. simiae OR1 MOI 0.1
N.D.
-0.0
+0.9
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.6
+0.7
-0.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.3
+1.1
-0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.5
+0.8
-0.0
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.8
+0.3
+0.1
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
+0.7
+0.6
+0.0
N.D.
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
+0.0
+1.1
+0.3
N.D.
carbon source
Carbon source D-Mannitol 10 mM
N.D.
+0.5
+0.8
+0.2
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+2.1
-0.4
-0.2
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
+0.5
+1.2
-0.1
N.D.
carbon source
L-Valine (C)
N.D.
+0.7
+0.6
+0.4
N.D.
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