Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01460 and PS417_01465 are separated by 123 nucleotidesPS417_01465 and PS417_01470 are separated by 348 nucleotidesPS417_01470 and PS417_01475 are separated by 207 nucleotidesPS417_01475 and PS417_01480 are separated by 240 nucleotides PS417_01460: PS417_01460 - tRNA-Thr, at 325,847 to 325,922 _01460 PS417_01465: PS417_01465 - hypothetical protein, at 326,046 to 326,279 _01465 PS417_01470: PS417_01470 - cell division protein FtsQ, at 326,628 to 327,578 _01470 PS417_01475: PS417_01475 - cell division protein FtsQ, at 327,786 to 328,706 _01475 PS417_01480: PS417_01480 - SAM-dependent methyltransferase, at 328,947 to 330,134 _01480
Group Condition PS417_01460 PS417_01465 PS417_01470 PS417_01475 PS417_01480
phage JP1 MOI 1 N.D. -0.6 -0.3 -0.1 -0.4
seeds Growth on radish seeds for 72 hours N.D. -0.3 +0.1 -1.0 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.6 -0.2 -0.3 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.7 -0.2 +0.2 -0.2
phage P. simiae OR1 MOI 0.1 N.D. +0.6 -0.3 -1.2 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 +0.0 -0.5 -0.7
supernatant control Vogels_fungal_media 0.1X N.D. +0.1 -0.5 +0.4 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.1 +0.1 +0.4 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 +0.3 -0.8 +0.4
carbon source L-Valine (C) N.D. +0.4 -0.4 -0.6 +0.2
seeds Growth on radish seeds for 24 hours N.D. -0.2 -0.2 +0.4 -0.4
carbon source L-Alanine (C) N.D. +0.2 -0.2 +0.3 -0.4
seeds Growth on radish seeds for 72 hours N.D. -0.6 -0.3 +0.5 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.1 -0.5 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.7 -0.1 -0.0 -0.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.4 +0.2 -0.4 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 +0.3 -0.4 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 -0.2 +0.1 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 +0.2 -0.1 +0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +0.5 -0.1 -0.4 +0.3
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) N.D. +0.5 -0.2 -0.4 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.5 +0.2 +0.1 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.9 +0.0 -0.2 -0.2
carbon source Carbon source L-Citrulline 5 mM N.D. -0.1 +0.3 +0.6 -0.2
motility outer cut, LB soft agar motility assay N.D. +0.4 +0.3 +0.3 -0.4
soil soil sample 2; outgrowth in LB N.D. +0.5 +0.3 -0.2 +0.1
supernatant control Vogels_fungal_media 0.1X N.D. +0.7 -0.2 +0.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.9 +0.2 -0.0 -0.0
phage P. simiae OR Antrim MOI 1 N.D. +0.6 +0.1 +0.3 +0.3
phage P. simiae ORA MOI 0.1 N.D. +0.4 +0.1 +0.6 +0.3
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