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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_14595 and PS417_14600 are separated by 116 nucleotides
PS417_14600 and PS417_14605 are separated by 147 nucleotides
PS417_14605 and PS417_14610 are separated by 86 nucleotides
PS417_14610 and PS417_14620 are separated by 444 nucleotides
PS417_14595: PS417_14595 - pyrroline-5-carboxylate reductase, at 3,191,365 to 3,192,138
_14595
PS417_14600: PS417_14600 - bleomycin resistance protein, at 3,192,255 to 3,192,611
_14600
PS417_14605: PS417_14605 - adenylate kinase, at 3,192,759 to 3,193,301
_14605
PS417_14610: PS417_14610 - lysine transporter LysE, at 3,193,388 to 3,194,008
_14610
PS417_14620: PS417_14620 - hypothetical protein, at 3,194,453 to 3,194,647
_14620
Group
Condition
PS417
_14595
PS417
_14600
PS417
_14605
PS417
_14610
PS417
_14620
rhizosphere
rhizosphere sample 8; outgrowth in LB
-1.4
-0.9
-0.4
-0.0
+0.1
seeds
Growth on radish seeds for 72 hours
-0.6
-1.0
-1.2
+0.7
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
-2.2
+0.4
-0.3
+0.0
soil
soil sample 5; outgrowth in LB
+0.1
+0.8
-1.9
-0.4
-0.4
soil
soil sample 6; outgrowth in LB
-0.2
-0.9
-0.2
+0.1
-0.8
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.7
-1.0
-0.4
+0.3
-0.0
carbon source
L-Valine (C)
-0.0
+0.4
-0.8
-0.7
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-1.6
+0.1
-0.5
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.6
+0.6
-1.3
+0.4
-0.4
phage
JP1 MOI 10
+0.1
-0.2
-1.1
+0.3
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-1.6
-0.1
-0.2
+0.2
seeds
Growth on radish seeds for 72 hours
-0.7
+0.4
-1.2
+0.4
+0.0
solid stress
Fraxetin 3 mM; solid stress
+0.3
-0.8
-0.8
+0.1
+0.2
motility
inner cut, LB soft agar motility assay
-0.4
+0.5
-1.1
+0.2
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.0
-0.5
-0.9
-0.1
+0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.5
-0.8
-0.2
+0.5
+1.1
motility
outer cut, LB soft agar motility assay
+0.1
+1.1
-1.0
+0.1
+0.0
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
+0.8
-0.9
-0.3
+0.6
carbon source
2'-Deoxyinosine 5 mM (C)
+1.2
-0.5
-0.3
+0.2
-0.2
stress
Phosphomycin 0.002 mg/ml
-0.7
+0.2
+0.8
-0.0
+0.3
phage
P. simiae Grant Run MOI 10
-0.4
+0.7
+0.8
-0.6
+0.4
stress
methylglyoxal 0.032 vol%
+0.5
-0.8
+0.3
+0.6
+0.2
phage
P. simiae ORA MOI 1
+0.8
+0.8
-0.3
-0.2
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.3
+1.2
+0.4
+0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.4
+1.5
-0.4
+0.2
-0.1
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
+0.9
-0.4
-0.0
+0.8
+0.3
phage
P. simiae OR Antrim MOI 1
+0.3
+0.6
+1.0
-0.5
+0.2
phage
P. simiae Grant Run MOI 0.1
+0.4
+0.9
+0.9
-0.3
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+2.1
+0.1
-0.2
-0.1
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
+0.8
+0.2
-0.1
+0.4
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